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Simultaneous detection and quantification of 3-nitrotyrosine and 3-bromotyrosine in human urine by stable isotope dilution liquid chromatography tandem mass spectrometry
- Source :
- Toxicology letters. 181(1)
- Publication Year :
- 2008
-
Abstract
- Nitration and bromination of proteins, giving rise to the respective 3-nitrotyrosine (3NT) and 3-bromotyrosine (3BT), are implicated in asthma, allergic inflammatory disorders, and cancer. We have developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (LC/MS/MS) assay for simultaneous analysis of protein-bound 3NT and 3BT in human urine. The detection limits (S/N=3) were 10 pg (44 fmol) for 3NT and 5.0 pg (19 fmol) for 3BT injected on-column. The average levels of protein-bound 3NT and 3BT in 23 healthy individuals were 9.7 +/- 11.0 (mean +/- S.D.) in 10(5) tyrosine and 4.4 +/- 3.9 (mean +/- S.D.) in 10(3) tyrosine, respectively, using this highly sensitive LC/MS/MS under the selective reaction monitoring mode. Furthermore, the levels of urinary 3NT and 3BT show a statistically significant correlation (R(2) = 0.55, p = 0.0065, n=23). The high specificity and accuracy of this LC/MS/MS method render it a valuable tool in measurement of 3NT and 3BrT in the human urinary protein as promising noninvasive biomarkers for protein tyrosine nitration and bromination in vivo.
- Subjects :
- Detection limit
Radioisotope Dilution Technique
Chromatography
Chemistry
General Medicine
Urine
Isotope dilution
Toxicology
Mass spectrometry
Tandem mass spectrometry
Sensitivity and Specificity
Liquid chromatography–mass spectrometry
Tandem Mass Spectrometry
Calibration
Humans
Tyrosine
Quantitative analysis (chemistry)
Chromatography, Liquid
Subjects
Details
- ISSN :
- 03784274
- Volume :
- 181
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Toxicology letters
- Accession number :
- edsair.doi.dedup.....9c183ae82e7e2d9613ac95b3f99eae16