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Mapping QTL for Resistance Against Viral Nervous Necrosis Disease in Asian Seabass
- Source :
- Marine Biotechnology. 18:107-116
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Viral nervous necrosis disease (VNN), caused by nervous necrosis virus (NNV), leads to mass mortality in mariculture. However, phenotypic selection for resistance against VNN is very difficult. To facilitate marker-assisted selection (MAS) for resistance against VNN and understanding of the genetic architecture underlying the resistance against this disease, we mapped quantitative trait loci (QTL) for resistance against VNN in Asian seabass. We challenged fingerlings at 37 days post-hatching (dph), from a single back-cross family, with NNV at a concentration of 9 × 10(6) TCID50/ml for 2 h. Daily mortalities were recorded and collected. A panel of 330 mortalities and 190 surviving fingerlings was genotyped using 149 microsatellites with 145 successfully mapped markers covering 24 linkage groups (LGs). Analysis of QTL for both resistance against VNN and survival time was conducted using interval mapping. Five significant QTL located in four LGs and eight suggestive QTL in seven LGs were identified for resistance. Another five significant QTL in three LGs and five suggestive QTL in three LGs were detected for survival time. One significant QTL, spanning 3 cM in LG20, was identified for both resistance and survival time. These QTL explained 2.2-4.1% of the phenotypic variance for resistance and 2.2-3.3% of the phenotypic variance for survival time, respectively. Our results suggest that VNN resistance in Asian seabass is controlled by many loci with small effects. Our data provide information for fine mapping of QTL and identification of candidate genes for a better understanding of the mechanism of disease resistance.
- Subjects :
- 0301 basic medicine
Candidate gene
Asia
Quantitative Trait Loci
Disease
Quantitative trait locus
Plant disease resistance
Applied Microbiology and Biotechnology
Fish Diseases
03 medical and health sciences
RNA Virus Infections
Animals
Genetic Predisposition to Disease
Nodaviridae
Survival rate
Disease Resistance
Genetics
biology
biology.organism_classification
Sea Bream
Genetic architecture
Survival Rate
030104 developmental biology
Genetic Loci
Microsatellite
Microsatellite Repeats
Subjects
Details
- ISSN :
- 14362236 and 14362228
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Marine Biotechnology
- Accession number :
- edsair.doi.dedup.....9c29acc025bd758be7f1c4fec0eb4b33