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Association of Matrix Metalloproteinase-2 (MMP-2) and MMP-9 Promoter Polymorphisms, Their Serum Levels, and Activities with Coronary Artery Calcification (CAC) in an Iranian Population

Authors :
Fariborz Bahrehmand
Nahid Salehi
Amir Kiani
Mohammadreza Saidi
Vahid Toupchi-Khosroshahi
Hadi Mozafari
Asad Vaisi Raygani
Daniel Elieh Ali Komi
Ehsan Mohammadi-Noori
saeed elahirad
Source :
Cardiovascular toxicology. 22(2)
Publication Year :
2021

Abstract

The serum levels and activity of matrix metalloproteinases (MMPs) are associated with the risk of coronary artery calcification (CAC). We sought to investigate the association between MMP-2 -1575GA (rs243866) and MMP-9 -1562 CT (rs3918242) SNPs with MMP-2 and MMP-9 serum levels and activity in individuals with CAC. One hundred and fifty-five cases with CAC and 155 healthy individuals as control group from West of Iran were included and frequency of genotypes and alleles of rs243866 and rs3918242 in MMP-2 and MMP-9 genes were determined using PCR-RFLP. We also investigated the serum levels of MMP-2 and MMP-9 and their activity using ELISA and gelatin zymography, respectively. Additionally, serum biochemical parameters including FBS (fasting blood sugar), urea, creatinine, cholesterol, triglyceride, HDL (high-density lipoprotein), LDL (low-density lipoprotein), calcium, and phosphorus as well as blood pressure (systolic blood pressure (SBP) and diastolic blood pressure (DBP)) were measured. Our results showed that both serum levels of MMP-2 and MMP-9 (P 0.001) and their activity (P 0.001) were higher in individuals with CAC when compared to the control group. Carrying A and T alleles in MMP-2 -1575GA (rs243866) and MMP-9 -1562 CT (rs3918242) SNPs, respectively, may predispose the individuals to CAC by acting as the risk factors. Serum levels and activity of MMP-2 and MMP-9 were found to be higher in CAC cases when compared to the healthy controls. Carriers of A allele in rs243866 SNP and T allele in rs3918242 SNP were shown to have higher MMP-2 and MMP-9 serum levels and activity that may result in increased ECM degradation and support the initiation and development of calcification.

Details

ISSN :
15590259
Volume :
22
Issue :
2
Database :
OpenAIRE
Journal :
Cardiovascular toxicology
Accession number :
edsair.doi.dedup.....9c6002ca32fcaba7162bb163a094b481