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Non-ionic cholesterol-based additives for the stabilization of membrane proteins

Authors :
Damien Cornut
Marine Soulié
Alexis Moreno
Gildas Nyame Mendendy Boussambe
Marjorie Damian
Sébastien Igonet
Pierre Guillet
Jean-Louis Banères
Grégory Durand
Source :
Biochimie.
Publication Year :
2022

Abstract

We report herein the synthesis of two non-ionic amphiphiles with a cholesterol hydrophobic moiety that can be used as chemical additives for biochemical studies of membrane proteins. They were designed to show a high similarity with the planar steroid core of cholesterol and small-to-medium polar head groups attached at the C3 position of ring-A on the sterol skeleton. The two Chol-Tris and Chol-DG have a Tris-hydroxymethyl and a branched diglucose polar head group, respectively, which provide them sufficient water solubility when mixed with the "gold standard" detergent n-Dodecyl-β-D-Maltoside (DDM). The colloidal properties of these mixed micelles were investigated by means of surface tension (SFT) measurements and dynamic light scattering (DLS) experiments and showed the formation of globular micelles of about 8 nm in diameter with a critical micellar concentration of 0.20 mM for DDM:Chol-DG and 0.22 mM for DDM:Chol-Tris. We showed that mixed micelles do not alter the extraction potency of a G-protein coupled receptor (GPCR): the human adenosine A2A receptor (A

Subjects

Subjects :
General Medicine
Biochemistry

Details

ISSN :
16386183
Database :
OpenAIRE
Journal :
Biochimie
Accession number :
edsair.doi.dedup.....9c6d1dfc674fcf12d546b6500b496874