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On-substrate Enzymatic Reaction to Determine Acetylcholinesterase Activity in Whole Blood by Paper Spray Mass Spectrometry
- Source :
- Journal of the American Society for Mass Spectrometry
- Publication Year :
- 2018
- Publisher :
- Springer US, 2018.
-
Abstract
- Currently, all assays measuring acetylcholinesterase (AChE) activity following a suspected nerve agent exposure leverage methodologies that fail to identify the agent. This limits the overall effectiveness and ability to administer proper countermeasures. As such, there is an urgent need to identify novel, rapid, and more comprehensive approaches to establish AChE activity, including identification of the toxicant. Paper spray mass spectrometry was used to monitor the activity of acetylcholinesterase, both in-solution and on modified hydrophobic paper surface. Hydrophobic paper surfaces were prepared using vaporized trichloro(3,3,3-trifluoropropyl)silane. In both approaches, mixtures of diluted human whole blood with and without VX were mixed with a non-endogenous AChE specific substrate, 1,1-dimethyl-4-acetylthiomethylpiperidinium (MATP+). Formation of the cleaved MATP+ product was monitored over time and compared to MATP+ to determine relative AChE activity. This on-substrate assay was effective at determining AChE activity and identifying the toxicant; however, determination of AChE activity in-solution proceeded at a slower rate. The on-substrate assay serves as a pioneering example of an enzymatic reaction occurring on the surface of a paper spray ionization ticket. This work broadens the range of applications relating to paper spray ionization-based clinical diagnostic assays. Graphical Abstractᅟ Electronic supplementary material The online version of this article (10.1007/s13361-018-2072-1) contains supplementary material, which is available to authorized users.
- Subjects :
- Paper
Aché
010402 general chemistry
Mass spectrometry
01 natural sciences
Acetylcholinesterase assay
VX
Mass Spectrometry
chemistry.chemical_compound
Piperidines
Structural Biology
medicine
Humans
Spectroscopy
Whole blood
Nerve agent
Enzyme Assays
chemistry.chemical_classification
Chromatography
Paper spray
010401 analytical chemistry
Acetylcholinesterase
language.human_language
0104 chemical sciences
Enzyme
chemistry
language
Substrate specificity
Toxicant
medicine.drug
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 18791123 and 10440305
- Volume :
- 29
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of the American Society for Mass Spectrometry
- Accession number :
- edsair.doi.dedup.....9c8ad6a42354caf9dc9b4b65edd03118