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Gene expression profiling of the long-term adaptive response to hypoxia in the gills of adult zebrafish
- Source :
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 289:R1512-R1519
- Publication Year :
- 2005
- Publisher :
- American Physiological Society, 2005.
-
Abstract
- Low oxygen levels (hypoxia) play a role in clinical conditions such as stroke, chronic ischemia, and cancer. To better understand these diseases, it is crucial to study the responses of vertebrates to hypoxia. Among vertebrates, some teleosts have developed the ability to adapt to extremely low oxygen levels. We have studied long-term adaptive responses to hypoxia in adult zebrafish. We used zebrafish that survived severe hypoxic conditions for 3 wk and showed adaptive behavioral and phenotypic changes. We used cDNA microarrays to investigate hypoxia-induced changes in expression of 15,532 genes in the respiratory organs (the gills). We have identified 367 differentially expressed genes of which 117 showed hypoxia-induced and 250 hypoxia-reduced expressions. Metabolic depression was indicated by repression of genes in the TCA cycle in the electron transport chain and of genes involved in protein biosynthesis. We observed enhanced expression of the monocarboxylate transporter and of the oxygen transporter myoglobin. The hypoxia-induced group further included the genes for Niemann-Pick C disease and for Wolman disease [lysosomal acid lipase (LAL)]. Both diseases lead to a similar intra- and extracellular accumulation of cholesterol and glycolipids. The Niemann-Pick C protein binds to cholesterol from internal lysosomal membranes and is involved in cholesterol trafficking. LAL is responsible for lysosomal cholesterol degradation. Our data suggest a novel adaptive mechanism to hypoxia, the induction of genes for lysosomal lipid trafficking and degradation. Studying physiological responses to hypoxia in species tolerant for extremely low oxygen levels can help identify novel regulatory genes, which may have important clinical implications.
- Subjects :
- Gills
medicine.medical_specialty
Time Factors
Physiology
government.form_of_government
Physiology (medical)
Internal medicine
Gene expression
medicine
Animals
Hypoxia
Zebrafish
Regulator gene
Niemann-Pick Diseases
Monocarboxylate transporter
biology
Reverse Transcriptase Polymerase Chain Reaction
Microarray analysis techniques
Gene Expression Profiling
Wolman Disease
Hypoxia (medical)
Microarray Analysis
biology.organism_classification
Adaptation, Physiological
Cell biology
Gene expression profiling
Endocrinology
Gene Expression Regulation
biology.protein
Lysosomal Lipase
government
medicine.symptom
Subjects
Details
- ISSN :
- 15221490 and 03636119
- Volume :
- 289
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Accession number :
- edsair.doi.dedup.....9c8bb9b63e62eca1a56ff4d6098c4251