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Different in vitro exposure regimens of murine primary macrophages to silver nanoparticles induce different fates of nanoparticles and different toxicological and functional consequences
- Source :
- Nanotoxicology, Nanotoxicology, Taylor & Francis, 2016, 10 (5), pp.586-596. ⟨10.3109/17435390.2015.1104738⟩, Nanotoxicology, 2016, 10 (5), pp.586-596. ⟨10.3109/17435390.2015.1104738⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- International audience; Silver nanoparticles (Ag-NPs) are used in a variety of consumers' goods. Their toxicological impact is currently intensely studied, mostly upon acute exposure, but their intracellular dissolution and fate is rather poorly documented. In this study, murine primary macrophages were exposed to a single high but non-lethal dose of Ag-NPs or to repeated, low doses of Ag-NPs. Cells were either collected immediately after acute exposure or after 72 h of recovery in the NP-free exposure medium. Ag intracellular content and distribution were analyzed by particle-induced X-ray emission, transmission electron microscopy coupled to energy-dispersive spectroscopy analysis and inductively coupled plasma mass spectrometry. In parallel, macrophage functionality as well as inflammatory and thiol-responses were assessed after Ag-NP exposure. We show that Ag accumulation in macrophages is similar upon acute and repeated exposure to Ag-NPs, and that Ag is partly expelled from cells during the 72 h recovery stage. However, acute exposure leads to a strong response of macrophages, characterized by reduced mitochondrial membrane potential, phagocytic capacity and nitric oxide (NO) production upon lipopolysaccharide (LPS) stimulation. Under this condition, we also show an increased release of proinflammatory cytokines as well as a decreased release of anti-inflammatory cytokines. This response is reversible since these biomarkers reach their basal level after the recovery phase; and is much less intense in repeatedly exposed cells. These results suggest that repeated exposure of macrophages to Ag-NPs, which is a more realistic exposure scenario than acute exposure, leads to significant Ag intracellular accumulation but a much less intense toxicological response
- Subjects :
- Lipopolysaccharides
Materials science
Silver
Lipopolysaccharide
Macrophage
Phagocytosis
Speciation
Primary Cell Culture
Biomedical Engineering
Metal Nanoparticles
Stimulation
02 engineering and technology
010501 environmental sciences
Toxicology
01 natural sciences
Proinflammatory cytokine
Nitric oxide
chemistry.chemical_compound
Mice
Nanoparticle
Microscopy, Electron, Transmission
Animals
Murine
0105 earth and related environmental sciences
Membrane Potential, Mitochondrial
Dose-Response Relationship, Drug
Toxicity
Macrophages
Spectrometry, X-Ray Emission
021001 nanoscience & nanotechnology
Cell biology
[SDV.TOX] Life Sciences [q-bio]/Toxicology
chemistry
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Immunology
Cytokines
0210 nano-technology
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 17435390 and 17435404
- Database :
- OpenAIRE
- Journal :
- Nanotoxicology, Nanotoxicology, Taylor & Francis, 2016, 10 (5), pp.586-596. ⟨10.3109/17435390.2015.1104738⟩, Nanotoxicology, 2016, 10 (5), pp.586-596. ⟨10.3109/17435390.2015.1104738⟩
- Accession number :
- edsair.doi.dedup.....9c90fc02f6d73a8b47d653cdd939e9ab