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Leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) is necessary for prostate cancer metastasis via epithelial-mesenchymal transition
- Source :
- The Journal of biological chemistry. 292(37)
- Publication Year :
- 2016
-
Abstract
- Prostate cancer is a highly penetrant disease among men in industrialized societies, but the factors regulating the transition from indolent to aggressive and metastatic cancer remain poorly understood. We found that men with prostate cancers expressing high levels of the G protein–coupled receptor LGR4 had a significantly shorter recurrence-free survival compared with patients with cancers having low LGR4 expression. LGR4 expression was elevated in human prostate cancer cell lines with metastatic potential. We therefore generated a novel transgenic adenocarcinoma of the mouse prostate (TRAMP) mouse model to investigate the role of Lgr4 in prostate cancer development and metastasis in vivo. TRAMP Lgr4−/− mice exhibited an initial delay in prostate intraepithelial neoplasia formation, but the frequency of tumor formation was equivalent between TRAMP and TRAMP Lgr4−/− mice by 12 weeks. The loss of Lgr4 significantly improved TRAMP mouse survival and dramatically reduced the occurrence of lung metastases. LGR4 knockdown impaired the migration, invasion, and colony formation of DU145 cells and reversed epithelial–mesenchymal transition (EMT), as demonstrated by up-regulation of E-cadherin and decreased expression of the EMT transcription factors ZEB, Twist, and Snail. Overexpression of LGR4 in LNCaP cells had the opposite effects. Orthotopic injection of DU145 cells stably expressing shRNA targeting LGR4 resulted in decreased xenograft tumor size, reduced tumor EMT marker expression, and impaired metastasis, in accord with our findings in TRAMP Lgr4−/− mice. In conclusion, we propose that Lgr4 is a key protein necessary for prostate cancer EMT and metastasis.
- Subjects :
- 0301 basic medicine
Male
Epithelial-Mesenchymal Transition
Lung Neoplasms
Mice, Transgenic
Biology
Adenocarcinoma
urologic and male genital diseases
Biochemistry
Metastasis
Receptors, G-Protein-Coupled
03 medical and health sciences
Prostate cancer
DU145
Prostate
Cell Movement
Cell Line, Tumor
LNCaP
medicine
Animals
Humans
Neoplasm Invasiveness
Epithelial–mesenchymal transition
Molecular Biology
Crosses, Genetic
Cell Proliferation
Mice, Knockout
Cancer
Prostatic Neoplasms
Molecular Bases of Disease
Cell Biology
medicine.disease
Survival Analysis
Recombinant Proteins
Neoplasm Proteins
Tumor Burden
030104 developmental biology
medicine.anatomical_structure
Immunology
Cancer research
RNA Interference
Neoplasm Transplantation
Tramp
Subjects
Details
- ISSN :
- 1083351X
- Volume :
- 292
- Issue :
- 37
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....9c9c8a15c92ae3c26b79f4985e456cc2