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Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2020
- Publisher :
- Oxford University Press, 2020.
-
Abstract
- This study uncovered a novel role for HCPro in ChiVMV pathogenicity by interacting with catalases and inhibiting their activities to counter plant immunity.<br />Plant symptoms are derived from specific interactions between virus and host components. However, little is known about viral or host factors that participate in the establishment of systemic necrosis. Here, we showed that helper component proteinase (HCPro), encoded by Chilli veinal mottle virus (ChiVMV), could directly interact with catalase 1 (CAT1) and catalase 3 (CAT3) in the cytoplasm of tobacco (Nicotiana tabacum) plants to facilitate viral infection. In vitro, the activities of CAT1 and CAT3 were inhibited by the interaction between HCPro and CATs. The C-terminus of HCPro was essential for their interaction and was also required for the decrease of enzyme activities. Interestingly, the mRNA and protein level of CATs were up-regulated in tobacco plants in response to ChiVMV infection. Nicotiana tabacum plants with HCPro overexpression or CAT1 knockout were more susceptible to ChiVMV infection, which was similar to the case of H2O2-pre-treated plants, and the overexpression of CAT1 inhibited ChiVMV accumulation. Also, neither CAT1 nor CAT3 could affect the RNA silencing suppression (RSS) activity of HCPro. Our results showed that the interaction between HCPro and CATs promoted the development of plant systemic necrosis, revealing a novel role for HCPro in virus infection and pathogenicity.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Nicotiana tabacum
Potyvirus
suppressor of RNA silencing
Plant Science
01 natural sciences
HCPro
Virus
03 medical and health sciences
Tobacco
systemic necrosis
Plant Diseases
Messenger RNA
biology
Host (biology)
AcademicSubjects/SCI01210
fungi
food and beverages
Hydrogen Peroxide
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Catalase
Virology
Research Papers
In vitro
RNA silencing
030104 developmental biology
Virus Diseases
Plant—Environment Interactions
Chilli veinal mottle virus
biology.protein
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14602431 and 00220957
- Volume :
- 71
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....9cb177bf6d486857f60eca244d8fd014