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Intestinal microbiota determines development of non-alcoholic fatty liver disease in mice
- Source :
- Gut, Gut, BMJ Publishing Group, 2013, 62 (12), pp.1787-1794. ⟨10.1136/gutjnl-2012-303816⟩, Gut 12 (62), 1787-1794. (2013), Gut, 2013, 62 (12), pp.1787-1794. ⟨10.1136/gutjnl-2012-303816⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Objective: Non-alcoholic fatty liver disease (NAFLD) is prevalent among obese people and is considered the hepatic manifestation of metabolic syndrome. However, not all obese individuals develop NAFLD. Our objective was to demonstrate the role of the gut microbiota in NAFLD development using transplantation experiments in mice. [br/] Design: Two donor C57BL/6J mice were selected on the basis of their responses to a high-fat diet (HFD). Although both mice displayed similar body weight gain, one mouse, called the responder', developed hyperglycaemia and had a high plasma concentration of pro-inflammatory cytokines. The other, called a non-responder', was normoglycaemic and had a lower level of systemic inflammation. Germ-free mice were colonised with intestinal microbiota from either the responder or the non-responder and then fed the same HFD. [br/] Results: Mice that received microbiota from different donors developed comparable obesity on the HFD. The responder-receiver (RR) group developed fasting hyperglycaemia and insulinaemia, whereas the non-responder-receiver (NRR) group remained normoglycaemic. In contrast to NRR mice, RR mice developed hepatic macrovesicular steatosis, which was confirmed by a higher liver concentration of triglycerides and increased expression of genes involved in de-novo lipogenesis. Pyrosequencing of the 16S ribosomal RNA genes revealed that RR and NRR mice had distinct gut microbiota including differences at the phylum, genera and species levels. [br/] Conclusions: Differences in microbiota composition can determine response to a HFD in mice. These results further demonstrate that the gut microbiota contributes to the development of NAFLD independently of obesity.
- Subjects :
- Blood Glucose
Male
[SDV]Life Sciences [q-bio]
Gut flora
Systemic inflammation
Polymerase Chain Reaction
Mice
Colonic Microflora
Cytokines
Fatty Liver
Real Time PCR
Lipid Metabolism
0302 clinical medicine
Non-alcoholic Fatty Liver Disease
RNA, Ribosomal, 16S
cytokine
0303 health sciences
biology
Microbiota
Fatty liver
Gastroenterology
Intestines
[SDV] Life Sciences [q-bio]
RNA, Bacterial
Liver
Lipogenesis
030211 gastroenterology & hepatology
medicine.symptom
medicine.medical_specialty
digestive system
03 medical and health sciences
stéatose hépatique
Internal medicine
medicine
Animals
Triglycerides
030304 developmental biology
pcr en temps réel
métabolisme lipidique
nutritional and metabolic diseases
Lipid metabolism
Fatty Acids, Volatile
medicine.disease
biology.organism_classification
Dietary Fats
Obesity
Mice, Inbred C57BL
Transplantation
Endocrinology
microflore digestive
Metabolic syndrome
Subjects
Details
- Language :
- English
- ISSN :
- 00175749 and 14683288
- Database :
- OpenAIRE
- Journal :
- Gut, Gut, BMJ Publishing Group, 2013, 62 (12), pp.1787-1794. ⟨10.1136/gutjnl-2012-303816⟩, Gut 12 (62), 1787-1794. (2013), Gut, 2013, 62 (12), pp.1787-1794. ⟨10.1136/gutjnl-2012-303816⟩
- Accession number :
- edsair.doi.dedup.....9cb60dec39d4711f9145d997e7cb2cd7