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In vitro characteristics of metastatic variant subclones of restricted genetic origin
- Source :
- Journal of Supramolecular Structure and Cellular Biochemistry. 15:139-151
- Publication Year :
- 1981
- Publisher :
- Wiley, 1981.
-
Abstract
- We have studied several metastatic variant cell lines derived from a common clonal origin and their transformed and untransformed parental cell lines. A number of in vitro characteristics were examined for each tumor line and these properties were correlated with the ability of the tumor cells to form pulmonary nodules in an experimental metastasis assay. Direct correlations with metastatic behavior in the lung colony assay were found to exist with the amount of cell-bound Concanavalin A and the procoagulant activities of cell lysates. In vitro parameters that did not correlate with the metastatic phenotype were: population doubling times in culture, saturation density achieved in culture, the number of colony-forming cells shed from confluent cultures, rates of cellular attachment to homotypic or heterotypic cell monolayers, plasminogen-activator production and procoagulant activity produced in serum-free conditioned medium.
- Subjects :
- Lung
Experimental metastasis
Cell
Metastatic phenotype
General Medicine
Biology
Biochemistry
Molecular biology
Phenotype
In vitro
Cell Line
Clone Cells
Plasminogen Activators
medicine.anatomical_structure
Cell culture
Concanavalin A
Immunology
Tumor Cells, Cultured
medicine
biology.protein
Animals
Neoplasm Metastasis
Subjects
Details
- ISSN :
- 15471748 and 02753723
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Journal of Supramolecular Structure and Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....9cc6c48725ada36ef2c538a6c45c4109