Association of Genetic Variants in miR-217 Gene with Risk of Coronary Artery Disease: A Case–Control Study

Xia Han,1,&ast; Xiaotang Liang,2,&ast; Menghai Wu,1 Lijun Zhang,1 Honglei Jiang2 1Department of Cardiology, Jinan People’s Hospital Affiliated to Shandong First Medical University, Laiwu, 271199, People’s Republic of China; 2Shandong Second Provincial General Hospital, Jinan, Shandong Province, 250000, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Honglei JiangShandong Second Provincial General Hospital, No. 4, Xingxi Street, Jinan, Shandong Province, 250000, People’s Republic of ChinaEmail jianghl_sd@21cn.comObjective: To evaluate the associations of genetic variants of the miR-217 gene with coronary artery disease (CAD) risk, as well as plasma level of vascular endothelial growth factor (VEGF).Methods: A case–control study with 498 CAD patients and 499 frequency-matched healthy controls was conducted to evaluate the associations of four tagSNPs of the miR-217 gene, including rs6724872, rs4999828, rs10206823, and rs41291177, with CAD risk and plasma level of VEGF.Results: SNP rs6724872 and rs4999828 were significantly associated with increased risk of CAD (P value was smaller than 0.05 even after Bonferroni multiple adjustment). Compared with the G allele, C allele of rs6724872 was significantly associated with 1.73-fold increased risk of CAD (95% CI: 1.25– 2.39; P = 0.001). While C allele of rs4999828 was significantly associated with 1.75-fold increased risk of CAD, compared with T allele (95% CI: 1.34– 2.29; P = 4 × 10− 5). Meanwhile, rs6724872 and rs4999828 were also significantly associated with higher level of VEGF (P < 0.001).Conclusion: These findings highlighted the important role of genetic variants of the miR-217 gene in the pathogenesis of CAD and potential targets for intervention.Keywords: coronary artery disease, miR-217, VEGF, genetic