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Molecular plasticity of E-cadherin and sialyl lewis x expression, in two comparative models of mammary tumorigenesis
- Source :
- PLoS ONE, Vol 4, Iss 8, p e6636 (2009), PLoS ONE
- Publication Year :
- 2009
- Publisher :
- Public Library of Science (PLoS), 2009.
-
Abstract
- BackgroundThe process of metastasis involves a series of steps and interactions between the tumor embolus and the microenvironment. Key alterations in adhesion molecules are known to dictate progression from the invasive to malignant phenotype followed by colonization at a distant site. The invasive phenotype results from the loss of expression of the E-cadherin adhesion molecule, whereas the malignant phenotype is associated with an increased expression of the carbohydrate ligand-binding epitopes, (e.g. Sialyl Lewis (x/a)) that bind endothelial E-selectin of the lymphatics and vasculature.MethodologyOur study analyzed the expression of two adhesion molecules, E-cadherin and Sialyl Lewis x (sLe(x)), in both a canine mammary carcinoma and human inflammatory breast cancer (IBC) model, using double labelled immunofluorescence staining.ResultsOur results demonstrate that canine mammary carcinoma and human IBC exhibit an inversely correlated cellular expression of E-cadherin and sLe(x) within the same tumor embolus.ConclusionsOur results in these two comparative models (canine and human) suggest the existence of a biologically coordinated mechanism of E-cadherin and sLe(x) expression (i.e. molecular plasticity) essential for tumor establishment and metastatic progression.
- Subjects :
- Pathology
medicine.medical_specialty
Science
Oligosaccharides
Biology
Immunofluorescence
Metastasis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Dogs
Cell Line, Tumor
medicine
Carcinoma
Animals
Humans
Sialyl Lewis X Antigen
Molecular Biology
030304 developmental biology
Canine Mammary Carcinoma
0303 health sciences
Multidisciplinary
medicine.diagnostic_test
Cell adhesion molecule
Cadherin
Mammary Neoplasms, Experimental
medicine.disease
Cadherins
Phenotype
Pathology/Molecular Pathology
3. Good health
Disease Models, Animal
Sialyl-Lewis X
Cell Transformation, Neoplastic
chemistry
030220 oncology & carcinogenesis
Oncology/Breast Cancer
Cancer research
Medicine
Female
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 4
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....9d309674041645a9450fdfd9ee9d7d69