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Isolation, Expression, and Chromosomal Localization of the Human Mitochondrial Capsule Selenoprotein Gene (MCSP)
- Source :
- Genomics. 32:184-190
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- The mitochondrial capsule selenoprotein (MCS) (HGMW-approved symbol MCSP) is one of three proteins that are important for the maintenance and stabilization of the crescent structure of the sperm mitochondria. We describe here the isolation of a cDNA, the exon-intron organization, the expression, and the chromosomal localization of the human MCS gene. Nucleotide sequence analysis of the human and mouse MCS cDNAs reveals that the 5'- and 3 '-untranslated sequences are more conserved (71%) than the coding sequences (59%). The open reading frame encodes a 116-amino-acid protein and lacks the UGA codons, which have been reported to encode the selenocysteines in the N-terminal of the deduced mouse protein. The deduced human protein shows a low degree of amino acid sequence identity to the mouse protein (39%). The most striking homology lies in the dicysteine motifs. Northern and Southern zooblot analyses reveal that the MCS gene in human, baboon, and bovine is more conserved than its counterparts in mouse and rat. The single intron in the human MCS gene is approximately 6 kb and interrupts the 5'-untranslated region at a position equivalent to that in the mouse and rat genes. Northern blot and in situ hybridization experiments demonstrate that the expression of the human MCS gene is restricted to haploid spermatids. The human gene was assigned to q21 of chromosome 1.
- Subjects :
- DNA, Complementary
Molecular Sequence Data
Mouse Protein
Biology
Homology (biology)
Mitochondrial Proteins
Open Reading Frames
03 medical and health sciences
0302 clinical medicine
Gene expression
Genetics
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Selenoproteins
Gene
In Situ Hybridization
030304 developmental biology
Regulation of gene expression
0303 health sciences
Base Sequence
Sequence Homology, Amino Acid
Genome, Human
Nucleic acid sequence
Chromosome Mapping
Proteins
Exons
Molecular biology
Introns
Open reading frame
Chromosomes, Human, Pair 1
030220 oncology & carcinogenesis
Human genome
Subjects
Details
- ISSN :
- 08887543
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Genomics
- Accession number :
- edsair.doi.dedup.....9d563931b50eeae1358da10736344652