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Reduced insulin binding to hepatic plasma membranes in D-galactosamine-treated rats
- Source :
- Diabetologia. 17(2)
- Publication Year :
- 1979
-
Abstract
- Six to 12 hr after IP injection of 400 mg/kg of D-galactosamine in rats a 5-fold increase in plasma insulin was observed. In addition, impaired glucose assimilation was present after an IV Load in spite of unchanged fasting glucose levels. In streptozotocin-diabetic rats (100 mg/kg IV) plasma insulin remained diminished 12 h after induction of D-galactosamine hepatitis. Under identical conditions of preparation and incubation, the liver plasma membranes of D-galactosamine-treated rats, in both normal and diabetic states, bound only 40--60% as much insulin per mg of membrane protein as those of the control rats. Scatchard analysis suggested that this was due to a decrease in the number of receptor sites in the membranes of the D-galactosamine-injected rats. No difference in the insulin degrading capacity and in insulin-receptor dissociation of the plasma membranes between control and D-galactosamine-treated groups was found. These data suggest that a reduction in the number of hepatic insulin receptors in galactosamine hepatitis can lead to insulin resistance and hyperinsulinaemia.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Galactosamine
chemistry.chemical_compound
Insulin resistance
Internal medicine
Internal Medicine
medicine
Animals
Insulin
Aspartate Aminotransferases
Receptor
Incubation
Hepatitis
biology
Chemistry
Cell Membrane
medicine.disease
Receptor, Insulin
Rats
Insulin receptor
Kinetics
Endocrinology
Membrane
Liver
biology.protein
Subjects
Details
- ISSN :
- 0012186X
- Volume :
- 17
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Diabetologia
- Accession number :
- edsair.doi.dedup.....9d61e765a55b4e11cbd22ec24658b342