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Direct Measurement of Single-Molecule Diffusion and Photodecomposition in Free Solution
- Source :
- Science. 275:1106-1109
- Publication Year :
- 1997
- Publisher :
- American Association for the Advancement of Science (AAAS), 1997.
-
Abstract
- Continuous monitoring of submillisecond free-solution dynamics of individual rhodamine-6G molecules and 30-base single-stranded DNA tagged with rhodamine was achieved. Fluorescence images were recorded from the same set of isolated molecules excited either through the evanescent field at the quartz-liquid interface or as a thin layer of solution defined by micron-sized wires, giving diffraction-limited resolution of interconnected attoliter volume elements. The single-molecule diffusion coefficients were smaller and the unimolecular photodecomposition lifetimes were longer for the dye-DNA covalent complex as compared with those of the dye molecule itself. Unlike bulk studies, stochastic behavior was found for individual molecules of each type, and smaller diffusion coefficients were observed.
- Subjects :
- Microscopy, Video
Photolysis
Multidisciplinary
Rhodamines
Photodissociation
Analytical chemistry
DNA, Single-Stranded
Fluorescence
Diffusion
Solutions
Rhodamine
chemistry.chemical_compound
chemistry
Chemical physics
Covalent bond
Excited state
Molecule
Diffusion (business)
Chemical decomposition
Fluorescent Dyes
Subjects
Details
- ISSN :
- 10959203 and 00368075
- Volume :
- 275
- Database :
- OpenAIRE
- Journal :
- Science
- Accession number :
- edsair.doi.dedup.....9d7f7f7fe74e0e9d377b9aa5ee4507a9