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High-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers
- Source :
- Cancer research. 66(8)
- Publication Year :
- 2006
-
Abstract
- Loss of DNA copy number at the short arm of chromosome 3 is one of the most common genetic changes in human lung cancer, suggesting the existence of one or more tumor suppressor genes (TSG) at 3p. To identify most frequently deleted regions and candidate TSGs within these regions, a recently developed single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG) technology was applied to investigate the loss of heterozygosity (LOH) in 30 primary non–small-cell lung cancers. A total of 386 SNP markers that spanned a region of 70 Mb at 3p, from 3pter to 3p14.1, were selected for LOH analysis. The average intermarker distance in the present study is ∼180 kb. Several frequently deleted regions, including 3p26.3, 3p25.3, 3p24.1, 3p23, and 3p21.1, were found. Several candidate TSGs within these frequently detected LOH regions have been found, including APG7L at 3p25.3, CLASP2 at 3p23, and CACNA2D3 at 3p21.1. This study also showed that SMSG technology is a very useful approach to rapidly define the minimal deleted region and to identify target TSGs in a given cancer. (Cancer Res 2006; 66(8): 4133-8)
- Subjects :
- Genetics
Cancer Research
Lung Neoplasms
Genotype
Cancer
Chromosome
Loss of Heterozygosity
Nucleic Acid Hybridization
Reproducibility of Results
Single-nucleotide polymorphism
Biology
medicine.disease
Polymorphism, Single Nucleotide
Loss of heterozygosity
Oncology
Chromosome 3
Polymorphism (computer science)
Carcinoma, Non-Small-Cell Lung
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
medicine
SNP
Humans
Genes, Tumor Suppressor
Chromosomes, Human, Pair 3
Subjects
Details
- ISSN :
- 00085472
- Volume :
- 66
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Cancer research
- Accession number :
- edsair.doi.dedup.....9d8cf2137040c2f6e969cf10583f1c4f