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RNA splicing capability of live neuronal dendrites
- Source :
- Proceedings of the National Academy of Sciences. 102:16859-16864
- Publication Year :
- 2005
- Publisher :
- Proceedings of the National Academy of Sciences, 2005.
-
Abstract
- Dendrites are specialized extensions of the neuronal soma that contain components of the cellular machinery involved in RNA and protein metabolism. Several dendritically localized proteins are associated with the precursor-mRNA (pre-mRNA) splicing complex, or spliceosome. Although some spliceosome-related, RNA-binding proteins are known to subserve separate cytoplasmic functions when moving between the nucleus and cytoplasm, little is known about the pre-mRNA splicing capacity of intact dendrites. Here, we demonstrate the presence and functionality of pre-mRNA-splicing components in dendrites. When isolated dendrites are transfected with a chicken δ-crystallin pre-mRNA or luciferase reporter pre-mRNA, splicing junctions clustered at or near expected splice sites are observed. Additionally, in vitro synaptoneurosome experiments show that this subcellular fraction contains a similar complement of splicing factors that is capable of splicing chicken δ-crystallin pre-mRNA. These observations suggest that pre-mRNA-splicing factors found in the dendroplasm retain the potential to promote pre-mRNA splicing.
- Subjects :
- Spliceosome
RNA Splicing
delta-Crystallins
In situ hybridization
Biology
Hippocampus
SR protein
RNA Precursors
medicine
Protein biosynthesis
Animals
In Situ Hybridization
Neurons
Multidisciplinary
RNA
Dendrites
Biological Sciences
Immunohistochemistry
Molecular biology
Rats
Cell biology
medicine.anatomical_structure
Cytoplasm
Protein Biosynthesis
RNA splicing
Chickens
Nucleus
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....9d9bb3bc59bf55ccfd9766e0d7c84034