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Combinatorial control of temporal gene expression in the Drosophila wing by enhancers and core promoters
- Source :
- BMC Genomics, O’Keefe, David D; Thomas, Sean R; Bolin, Kelsey; Griggs, Ellen; Edgar, Bruce A; & Buttitta, Laura A. (2012). Combinatorial control of temporal gene expression in the Drosophila wing by enhancers and core promoters. BMC Genomics, 13(1), 498. doi: http://dx.doi.org/10.1186/1471-2164-13-498. Retrieved from: http://www.escholarship.org/uc/item/3dx1x6bj
- Publication Year :
- 2012
-
Abstract
- Background The transformation of a developing epithelium into an adult structure is a complex process, which often involves coordinated changes in cell proliferation, metabolism, adhesion, and shape. To identify genetic mechanisms that control epithelial differentiation, we analyzed the temporal patterns of gene expression during metamorphosis of the Drosophila wing. Results We found that a striking number of genes, approximately 50% of the Drosophila transcriptome, exhibited changes in expression during a time course of wing development. While cis-acting enhancer sequences clearly correlated with these changes, a stronger correlation was discovered between core-promoter types and the dynamic patterns of gene expression within this differentiating tissue. In support of the hypothesis that core-promoter type influences the dynamics of expression, expression levels of several TATA-box binding protein associated factors (TAFs) and other core promoter-associated components changed during this developmental time course, and a testes-specific TAF (tTAF) played a critical role in timing cellular differentiation within the wing. Conclusions Our results suggest that the combinatorial control of gene expression via cis-acting enhancer sequences and core-promoter types, determine the complex changes in gene expression that drive morphogenesis and terminal differentiation of the Drosophila wing epithelium.
- Subjects :
- Cellular differentiation
Terminal differentiation
Morphogenesis
Gene Expression
Microarray
03 medical and health sciences
0302 clinical medicine
RNA interference
Gene expression
Genetics
Cell Adhesion
Animals
Drosophila Proteins
Wings, Animal
RNA, Small Interfering
Enhancer
Promoter Regions, Genetic
Gene
Core promoter
030304 developmental biology
Cell Proliferation
Cis-regulatory sequence
Cell cycle exit
0303 health sciences
biology
Metamorphosis
Gene Expression Regulation, Developmental
Cell Differentiation
biology.organism_classification
Wing Morphogenesis
Drosophila melanogaster
Enhancer Elements, Genetic
TATA Box Binding Protein-Like Proteins
RNA Interference
Transcriptome
TATA box binding protein-associated factor
030217 neurology & neurosurgery
Drosophila Protein
Biotechnology
Research Article
Subjects
Details
- ISSN :
- 14712164
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- BMC genomics
- Accession number :
- edsair.doi.dedup.....9dc18206b3fb7277b7dd9cee4b6cf1dc