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Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation
- Source :
- Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- To investigate the effect of P2 receptor on microglia and its inhibitor PPADS on choroidal neovascularization. Forty CX3CR1GFP/+ mice were randomly divided into 8 groups. In addition to the normal group, the rest of groups were receiving laser treatment. The retina and choroid from the second, third, fourth and fifth group of mice were taken in the 1, 4, 7, 14 days after laser treatment. The mice in the sixth and seventh group received intravitreal injection of 2 µl PPADS or PBS respectively immediately after laser treatment. The mice in the eighth group received topical application of PPADS once per day of three days. The mice in sixth, seventh and eighth group received AF and FFA examination on the fourth day after laser treatment. Immunofluorescence histochemical staining and real-time quantitative PCR were used to evaluate P2 expression and its effect on choroidal neovascularization. After laser treatment, activated microglia can express P2 receptors (P2X4, P2X7, P2Y2 and P2Y12). The expression of P2 increased on the first day after laser damage, peaked on the fourth day (tP2X4 = 6.05, tP2X7 = 2.95, tP2Y2 = 3.67, tP2Y12 = 5.98, all P P2X4 = 5.54, tP2X7 = 9.82, tP2Y2 = 3.86, tP2Y12 = 7.91, all P P2X4 = 3.24, tP2X7 = 5.89, tP2Y2 = 6.75, tP2Y12 = 4.97, all P
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Science
P2 receptor
Article
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
P2Y12
Internal medicine
Purinergic P2 Receptor Antagonists
medicine
Animals
PPADS
Receptor
Eye diseases
Multidisciplinary
Microglia
Receptors, Purinergic P2
business.industry
Lasers
Purinergic receptor
Choroidal Neovascularization
Experimental models of disease
030104 developmental biology
medicine.anatomical_structure
Choroidal neovascularization
Endocrinology
chemistry
Pyridoxal Phosphate
Medicine
Choroid
medicine.symptom
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....9dec028aa86189782d9f1cdbb2afc98b