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Laparoscopic surgery for early cervical squamous cell carcinoma and its effect on the micrometastasis of cancer cells
- Source :
- Medicine
- Publication Year :
- 2018
- Publisher :
- Wolters Kluwer Health, 2018.
-
Abstract
- The aim of this study was to evaluate the effect of laparoscopic radical hysterectomy on expressions of circulating tumor cells (CTCs) of cytokeratin 19 (CK19), cytokeratin 20 (CK20), and squamous cell carcinoma antigen (SCC-Ag) mRNA. We collect 78 patients with stage IA2-IIA1 cervical cancer who underwent radical hysterectomy by laparotomy or laparoscopy in our study, and 34 uterine fibroids patients and 32 healthy subjects were recruited as the positive control group and negative control group, respectively. Blood samples were taken from early-stage primary cervical squamous cell carcinoma patients. Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to amplify peripheral blood CK19, CK20 and SCC-Ag from total RNA. We measured the expression of CK19, CK20, and SCC-Ag before laparoscopic radical hysterectomy, 24 hours and 30 days after surgery. Meanwhile, the expression of these markers was compared between laparoscopic and laparotomy groups. The expressions of CK19, CK20, and SCC-Ag in the experimental group before surgery were (0.0035 ± 0.0018), (1.06 ± 0.49), and (1.48 ± 0.46), respectively, and the positive rates were 32.1%, 33.3%, and 35.9%, respectively. The expression levels of CK19, CK20, and SCC-Ag in the experimental group before surgery was significantly higher than the positive and negative control groups, and there were no significant differences between the positive and negative control groups. The expressions and positive rates of CK19, CK20, and SCC-Ag before laparoscopic radical hysterectomy were significantly lower than the stage at 24 hours after surgery (P .05). There were no significant differences in CK19, CK20, and SCC-Ag expressions before surgery, 24 hours and 30 days after surgery between laparoscopic group and laparotomy group (P > .05). Both laparotomy and laparoscopic radical mastectomy tend to increase the expression of CTCs in peripheral blood, and the expressions have no differences between these 2 groups. So, the use of CK19, CK20, and SCC-Ag expression levels from peripheral blood from early stage cervical cancer radical patients before hysterectomy can aid to overcome the lack of radiographic examination and tumor markers measurement, and provide clues for postoperative treatment and prognosis determination.
- Subjects :
- 0301 basic medicine
Laparoscopic surgery
Adult
medicine.medical_specialty
cervical cancer
medicine.medical_treatment
squamous cell carcinoma antigen
Urology
Observational Study
Down-Regulation
Uterine Cervical Neoplasms
Keratin-20
circulating tumor cells
Hysterectomy
03 medical and health sciences
0302 clinical medicine
Circulating tumor cell
laparoscopic radical hysterectomy
Antigens, Neoplasm
Laparotomy
Carcinoma
Medicine
Humans
Radical Hysterectomy
cytokeratin 19
Radical mastectomy
Serpins
Cervical cancer
Keratin-19
business.industry
cytokeratin 20
General Medicine
real-time reverse transcription polymerase chain reaction (qRT-PCR)
Middle Aged
medicine.disease
Gene Expression Regulation, Neoplastic
030104 developmental biology
Treatment Outcome
Neoplasm Micrometastasis
030220 oncology & carcinogenesis
Case-Control Studies
Carcinoma, Squamous Cell
Female
Laparoscopy
business
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 15365964 and 00257974
- Volume :
- 97
- Issue :
- 34
- Database :
- OpenAIRE
- Journal :
- Medicine
- Accession number :
- edsair.doi.dedup.....9df8e43a2605677def95d3e2eddc2930