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Different Plasmodium falciparum Recombinant MSP119Antigens Differ in Their Capacities to Stimulate In Vitro Peripheral Blood T Lymphocytes in Individuals from Various Endemic Areas
- Source :
- Scandinavian Journal of Immunology. 49:431-440
- Publication Year :
- 1999
- Publisher :
- Wiley, 1999.
-
Abstract
- This study reports on T-cell proliferative responses to the 19-kDa C-terminal domain of the Plasmodium falciparum merozoite surface protein (MSP1(19)). Three different recombinant proteins were used: an Escherichia coli product expressing the first EGF-like domain and Saccharomyces cerevisiae and baculovirus/insect-cell-produced proteins containing both EGF-like domains, the latter protein being produced with or without N-glycosylation. Cell donors were P. falciparum-immune adults with no recent history of clinical malaria and recruited from three Senegalese settings with different epidemiological parasite transmission. Each mononuclear-blood-cell preparation was stimulated with a range of concentrations of the three proteins. Most subjects' mononuclear cells were reactive to at least one protein, but significant differences in lymphoproliferation were seen between the settings and within individual cultures depending on the protein source and concentration. Importantly, lymphoproliferation indices correlated inversely with the intensity of P. falciparum malaria transmission. When purified T lymphocytes were cultured in the presence of MSP1(19) plus autologous monocytes, B lymphocytes or a proposed CD1+ dendritic-cell population as costimulatory cells, significant differences were observed depending on the individual's previous exposure to parasites. This study shows that the stimulation of lymphocyte proliferation in vitro with MSP1(19) depends on several factors, including epidemiological conditions and protein preparations.
- Subjects :
- Adult
Male
Endemic Diseases
Plasmodium falciparum
Immunology
Population
CD1
Antibodies, Protozoan
Antigen-Presenting Cells
Lymphocyte proliferation
Biology
Lymphocyte Activation
Peripheral blood mononuclear cell
law.invention
Interferon-gamma
Antigen
T-Lymphocyte Subsets
law
parasitic diseases
Animals
Humans
Malaria, Falciparum
education
Cells, Cultured
Merozoite Surface Protein 1
education.field_of_study
Autologous Monocytes
General Medicine
Middle Aged
biology.organism_classification
Peptide Fragments
Recombinant Proteins
Senegal
Molecular Weight
Immunoglobulin G
Recombinant DNA
Female
Subjects
Details
- ISSN :
- 13653083 and 03009475
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Scandinavian Journal of Immunology
- Accession number :
- edsair.doi.dedup.....9dfb9d0dcbc7d056b3a7a6ce0b226de4