Back to Search
Start Over
A clinical‐grade acellular matrix for esophageal replacement
- Source :
- Journal of Tissue Engineering and Regenerative Medicine, Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2019, 13 (12), pp.2191-2203. ⟨10.1002/term.2983⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- In pathologies of the esophagus such as esophageal atresia, cancers, and caustic injuries, methods for full thickness esophageal replacement require the sacrifice of healthy intra-abdominal organs such as the stomach and the colon and are associated with high morbidity, mortality, and poor functional results. To overcome these problems, tissue engineering methods are developed to create a substitute with scaffolds and cells. The aim of this study was to develop a simple and safe decellularization process in order to obtain a clinical grade esophageal extracellular matrix. Following the decontamination step, porcine esophagi were decellularized in a bioreactor with sodium dodecyl sulfate and ethylenediaminetetraacetic acid for 3 days and were rinsed with deionized water. DNA was eliminated by a 3-hr DNase treatment. To remove any residual detergent, the matrix was then incubated with an absorbing resin. The resulting porcine esophageal matrix was characterized by the assessment of the efficiency of the decellularization process (DNA quantification), evaluation of sterility and absence of cytotoxicity, and its composition and biomechanical properties, as well as the possibility to be reseeded with mesenchymal stem cells. Complete decellularization with the preservation of the general structure, composition, and biomechanical properties of the native esophageal matrix was obtained. Sterility was maintained throughout the process, and the matrix showed no cytotoxicity. The resulting matrix met clinical grade criteria and was successfully reseeded with mesenchymal stem cells..
- Subjects :
- Pathology
medicine.medical_specialty
Swine
0206 medical engineering
Biomedical Engineering
Medicine (miscellaneous)
02 engineering and technology
Matrix (biology)
Biomaterials
Extracellular matrix
03 medical and health sciences
chemistry.chemical_compound
Esophagus
Tissue engineering
Materials Testing
medicine
Animals
[CHIM]Chemical Sciences
Sodium dodecyl sulfate
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
[PHYS]Physics [physics]
0303 health sciences
Decellularization
Tissue Engineering
Tissue Scaffolds
Chemistry
Stomach
Mesenchymal stem cell
Mesenchymal Stem Cells
020601 biomedical engineering
Extracellular Matrix
medicine.anatomical_structure
Subjects
Details
- Language :
- English
- ISSN :
- 19327005 and 19326254
- Database :
- OpenAIRE
- Journal :
- Journal of Tissue Engineering and Regenerative Medicine, Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2019, 13 (12), pp.2191-2203. ⟨10.1002/term.2983⟩
- Accession number :
- edsair.doi.dedup.....9e0d5784be26bceb17b771b0b3ed9f8e