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Imbalance between circulating CD4+regulatory T and conventional T lymphocytes in patients with HBV-related acute-on-chronic liver failure
- Source :
- Liver International. 33:1517-1526
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- Background & Aims The important pathophysiological role of immune dysfunction, especially innate immune dysfunction in patients with acute-on-chronic liver failure (ACLF), has been investigated in recent years, but dysregulation of adaptive immunity remains poorly elucidated. The aim of this study was to (i) determine the CD3+ T-lymphocyte count and the balance between CD4+ regulatory T (Tregs) and conventional T cells (Tconv) in hepatitis B virus (HBV)-related ACLF patients; (ii) analyse the frequencies of Tregs subpopulations; and (iii) assess the suppressive potency of CD4+ Tregs and each fraction. Methods We enrolled 20 HBV-ACLF patients, 10 septic shock subjects, 20 chronic hepatitis B (CHB) patients and 20 healthy volunteers (HC). Based on flow cytometry, we performed the absolute counting of circulating T lymphocytes and phenotyping of CD4+ Tregs and quantified the effects of Tregs and each subpopulation on Tconv proliferation by CFSE staining. Results Compared with CHB patients and HC, we observed an equal reduction in peripheral T subsets in HBV-ACLF and septic shock subjects; the number of CD4+ Tregs remained unchanged and the Tconv count declined, promoting elevation of the Treg-to-Tconv ratio. The frequencies of Treg-II and -III were elevated in HBV-ACLF. Functional studies showed that the suppressive capacity of Tregs was preserved in the HBV-ACLF group and Treg-II came first. Conclusions Similar to septic shock subjects, in HBV-ACLF patients there exists a reduction in CD4+ T lymphocytes, predominantly CD4+ Tconv, and the development of suppressive CD4+ Tregs greatly prevails over Tconv, constituting important characteristics of adaptive immune dysfunction of HBV-ACLF.
- Subjects :
- Adult
Male
China
CD3
Succinimides
chemical and pharmacologic phenomena
medicine.disease_cause
T-Lymphocytes, Regulatory
Immunophenotyping
Flow cytometry
End Stage Liver Disease
Hepatitis B, Chronic
T-Lymphocyte Subsets
medicine
Humans
Potency
Hepatitis B virus
Analysis of Variance
Innate immune system
Hepatology
biology
medicine.diagnostic_test
business.industry
Septic shock
Liver Failure, Acute
Middle Aged
Flow Cytometry
Fluoresceins
medicine.disease
Acquired immune system
Shock, Septic
Pathophysiology
Immunology
biology.protein
Female
business
Subjects
Details
- ISSN :
- 14783223
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Liver International
- Accession number :
- edsair.doi.dedup.....9e3015e8255deafcdf29199902326889