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Native Adipose Stromal Cells (ASCs) Egress from Adipose Tissue in vivo: Evidence During Lymph Node Activation
- Source :
- Stem Cells / Stem Cells (Miamisburg), Stem Cells / Stem Cells (Miamisburg), 2013, epub ahead of print. ⟨10.1002/stem.1375⟩, Stem Cells / Stem Cells (Miamisburg), Alphamed Press, 2013, epub ahead of print. ⟨10.1002/stem.1375⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Adipose tissue (AT) has become accepted as a source of multipotent progenitor cells, the adipose stromal cells (ASCs). In this regard, considerable work has been performed to harvest and characterize this cell population as well as to investigate the mechanisms by which transplanted ASCs mediate tissue regeneration. In contrast the endogenous release of native ASCs by AT has been poorly investigated. In this work, we show that native ASCs egress from murine AT. Indeed, we demonstrated that the release of native ASCs from AT can be evidenced both using an ex vivo perfusion model that we set up and in vivo. Such a mobilization process is controlled by CXCR4 chemokine receptor. In addition, once mobilized from AT, circulating ASCs were found to navigate through lymph fluid and to home into lymph nodes (LN). Therefore, we demonstrated that, during the LN activation, the fat depot encapsulating the activated LN releases native ASCs, which in turn invade the activated LN. Moreover, the ASCs invading the LN were visualized in close physical interaction with podoplanin and ER-TR7 positive structures corresponding to the stromal network composing the LN. This dynamic was impaired with CXCR4 neutralizing antibody. Taken together, these data provide robust evidences that native ASCs can traffic in vivo and that AT might provide stromal cells to activated LNs.
- Subjects :
- Male
Receptors, CXCR4
Stromal cell
Population
Adipose tissue
Cell Growth Processes
Biology
Immunophenotyping
03 medical and health sciences
Chemokine receptor
Mice
0302 clinical medicine
In vivo
medicine
Animals
Progenitor cell
education
Lymph node
030304 developmental biology
0303 health sciences
education.field_of_study
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
hemic and immune systems
Cell Biology
eye diseases
Chemokine CXCL12
Cell biology
Mice, Inbred C57BL
medicine.anatomical_structure
Adipose Tissue
030220 oncology & carcinogenesis
Immunology
Molecular Medicine
Lymph Nodes
tissues
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 15494918
- Database :
- OpenAIRE
- Journal :
- Stem Cells / Stem Cells (Miamisburg), Stem Cells / Stem Cells (Miamisburg), 2013, epub ahead of print. ⟨10.1002/stem.1375⟩, Stem Cells / Stem Cells (Miamisburg), Alphamed Press, 2013, epub ahead of print. ⟨10.1002/stem.1375⟩
- Accession number :
- edsair.doi.dedup.....9e501ed0292f8a507e36220f0849f30b