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Characterization of the Escherichia coli YajL, YhbO and ElbB glyoxalases
- Source :
- FEMS microbiology letters. 363(3)
- Publication Year :
- 2015
-
Abstract
- The DJ-1 superfamily is a group of proteins that shares a similarity with the human DJ-1, known to be associated with Parkinson disease. Novel glyoxalase activity, converting α-oxoaldehydes to carboxylic acids, has been reported for DJ-1 homologs in humans, worms, plants, and bacteria. The four Escherichia coli genes, hchA , yajL , yhbO and elbB , have been known to share sequence similarities and catalytic residues with other DJ-1 superfamily members. We investigated here whether they exhibit similar glyoxalase activity, as previously shown for HchA protein. Purified YajL, YhbO and ElbB exhibited glyoxalase activity with different substrate specificities, optimal pHs, and metal effects. Overexpressions of the homologs enhance cellular protection from exogenously added glyoxals and reduce the glyoxal-dependent increase in intracellular advanced glycation end products (AGEs). Based on their expression, primarily during the stationary phase, we speculate that their roles in cells as glyoxalases are manifested during the stationary phase.
- Subjects :
- 0301 basic medicine
Ribosomal Proteins
Glycosylation
Protein DJ-1
Hydrolases
Enzyme Activators
medicine.disease_cause
Microbiology
Substrate Specificity
03 medical and health sciences
chemistry.chemical_compound
Glycation
Genetics
medicine
Escherichia coli
Enzyme Inhibitors
Molecular Biology
Gene
Heat-Shock Proteins
biology
Escherichia coli Proteins
Methylglyoxal
Glutathione
Glyoxal
Hydrogen-Ion Concentration
biology.organism_classification
030104 developmental biology
chemistry
Biochemistry
Metals
Bacteria
Subjects
Details
- ISSN :
- 15746968
- Volume :
- 363
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- FEMS microbiology letters
- Accession number :
- edsair.doi.dedup.....9e5be11265b81681f650b0d5472510b7