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Monoclonal antibodies against vascular endothelial growth factor 165 (VEGF165): neutralization of biological activity and recognition of the epitope
- Source :
- Biochemistry and molecular biology international. 47(2)
- Publication Year :
- 1999
-
Abstract
- Five antibodies against vascular endothelial growth factor 165 (VEGF165) were obtained. These antibodies, Ab-2, Ab-20, Ab-153, Ab-309, Ab-342, were able to recognize not only native VEGF165, but also reducing VEGF165. Three of the antibodies, Ab-153, Ab-309, Ab-342, were identified as VEGF165 neutralizing antibody on the basis of its ability to inhibit the proliferation of human umbilical vein endothelial cells (HUVEC) induced by VEGF165 and the binding of [125I]-VEGF165 to receptors on HUVEC. The fragments of E1 (VEGF120-135) and E2 (VEGF46-60) recognized by neutralizing antibodies may be related the receptor binding domain of VEGF.
- Subjects :
- Vascular Endothelial Growth Factor A
medicine.drug_class
Clinical Biochemistry
Molecular Sequence Data
Endothelial Growth Factors
Monoclonal antibody
Biochemistry
Binding, Competitive
Epitope
Umbilical vein
Epitopes
Mice
Genetics
medicine
Animals
Receptors, Growth Factor
Amino Acid Sequence
Neutralizing antibody
Receptor
Molecular Biology
Cells, Cultured
Lymphokines
biology
Chemistry
Vascular Endothelial Growth Factors
Antibodies, Monoclonal
Receptor Protein-Tyrosine Kinases
Biological activity
Cell Biology
Molecular biology
Peptide Fragments
Vascular endothelial growth factor A
Receptors, Vascular Endothelial Growth Factor
biology.protein
Antibody
Cell Division
Protein Binding
Subjects
Details
- ISSN :
- 10399712
- Volume :
- 47
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemistry and molecular biology international
- Accession number :
- edsair.doi.dedup.....9e6ed3421fb2e9c181d568e36fdba1e6