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Microbial community structure and functional diversity of nitrogen-fixing bacteria associated with Colophospermum mopane
- Source :
- FEMS Microbiology Ecology. 91
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- Colophospermum mopane is an indigenous legume tree that grows in Southern Africa and is one of the predominant trees of the woodland vegetation. In order to increase knowledge about its ecology, especially how C. mopane thrives in the nitrogen-poor soils of the region, we analyzed the root-associated bacteria to assess the active diazotrophic diversity and total microbial diversity by culture-dependent and independent techniques. Root nodules were not detected but in some samples the lateral roots showed an outgrowth-like protuberance, that were not likely to have functions related to legume root nodules. The bacterial isolates recovered were related to Actinobacteria, Firmicutes and Proteobacteria. The total microbial diversity was dominated by Actinobacteria-related phylotypes, while the active diazotrophic diversity showed that the majority of the sequences were related to the order Rhizobiales but also to Spirochaetes, Firmicutes, Bacteroidetes and Deltaproteobacteria. Several isolates showed characteristics of plant growth-promoting bacteria. These findings increase the spectrum of possible phylotypes that can be found in legume trees that are typically nodulated by Alpha- and Betaproteobacteria, and reveal for the first time a surprising diversity of nitrogen-fixing bacteria active in legume tree roots.
- Subjects :
- DNA, Bacterial
Root nodule
Firmicutes
Mopane
Deltaproteobacteria
Plant Roots
Applied Microbiology and Biotechnology
Microbiology
Africa, Southern
Actinobacteria
Soil
Nitrogen Fixation
RNA, Ribosomal, 16S
Proteobacteria
Botany
Soil Microbiology
Betaproteobacteria
Base Sequence
Ecology
biology
Bacteroidetes
Fabaceae
Biodiversity
Sequence Analysis, DNA
biology.organism_classification
Bacterial Typing Techniques
human activities
Subjects
Details
- ISSN :
- 15746941
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Ecology
- Accession number :
- edsair.doi.dedup.....9e847fa488b5a2a4bf2a2427ab09285b
- Full Text :
- https://doi.org/10.1093/femsec/fiv030