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Sensitive and selective fluorescent probe for hypochlorite in 100% aqueous solution and its application for lysosome-targetable cell imaging
- Source :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 231
- Publication Year :
- 2019
-
Abstract
- A morpholine-functionalized pyrrole-cyanine probe was synthesized via a simple condensation reaction in high yield. This probe exhibits high selectivity toward ClO− on fluorescence and UV–vis spectra in neat aqueous solution. The strong green emission of the probe solution was quenched and the yellow color faded immediately upon the addition of ClO−. The detection limit of the probe for ClO− was 0.165 μM. The mechanism of hypochlorite-induced C C breakage was supposed on the basis of EIS-MS, NMR, and density functional theory (DFT) calculation. Finally, the probe was utilized to image ClO− in lysosomes of living cells.
- Subjects :
- Models, Molecular
Hypochlorite
02 engineering and technology
010402 general chemistry
Photochemistry
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
Lysosome
medicine
Humans
Instrumentation
Spectroscopy
Fluorescent Dyes
Detection limit
Aqueous solution
Microscopy, Confocal
Optical Imaging
Water
021001 nanoscience & nanotechnology
Condensation reaction
Fluorescence
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Hypochlorous Acid
medicine.anatomical_structure
Spectrometry, Fluorescence
chemistry
Solubility
Yield (chemistry)
Density functional theory
0210 nano-technology
Lysosomes
HeLa Cells
Subjects
Details
- ISSN :
- 18733557
- Volume :
- 231
- Database :
- OpenAIRE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Accession number :
- edsair.doi.dedup.....9eb2880cf587806f6a7bc00ebcbb7b96