Back to Search
Start Over
Synergistic inhibitory effects of capsaicin combined with cisplatin on human osteosarcoma in culture and in xenografts
- Source :
- Journal of Experimental & Clinical Cancer Research, Vol 37, Iss 1, Pp 1-17 (2018), Journal of Experimental & Clinical Cancer Research : CR
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Background The combination of phytochemicals with chemotherapy drugs is an emerging new strategy for cancer therapy to increase antitumor responses. Methods The present study investigates the effect of the combination of capsaicin (CAP) with cisplatin (DDP) and the potential underlying anticancer mechanisms in osteosarcoma (OS) cells in vitro and in vivo. Results Cell viability assays and isobolographic analyses demonstrated that the combination of CAP and DDP showed synergistic cytotoxic effects on OS cells. We chose relatively low concentrations of CAP (100 μM) and DDP (16.7 μM) for subsequent experiments. Generally, the combination of CAP and DDP had significant effects on apoptosis induction, cell cycle arrest and cell invasion inhibition in OS cells compared with the individual-treatment groups and the control group. Moreover, cotreatment with CAP and DDP triggered prosurvival autophagy through reactive oxygen species (ROS)/JNK and p-AKT/mTOR signaling in OS cells. The combination regimen of CAP and DDP also inhibited tumor growth in an OS xenograft model. Conclusion These results suggest that the combination of CAP and DDP has strong inhibitory effects on OS cells and identify CAP as a promising agent for supplementing standard chemotherapy and possible future targeted therapy in OS.
- Subjects :
- Male
0301 basic medicine
Cancer Research
Cell cycle checkpoint
endocrine system diseases
Combination therapy
medicine.medical_treatment
Mice, Nude
Bone Neoplasms
Apoptosis
lcsh:RC254-282
Targeted therapy
Mice
03 medical and health sciences
0302 clinical medicine
In vivo
Cell Line, Tumor
Antineoplastic Combined Chemotherapy Protocols
Autophagy
medicine
Animals
Humans
Cytotoxic T cell
Viability assay
Cisplatin
Osteosarcoma
Chemistry
Research
Drug Synergism
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Xenograft Model Antitumor Assays
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer research
Capsaicin
Reactive Oxygen Species
medicine.drug
Subjects
Details
- ISSN :
- 17569966
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental & Clinical Cancer Research
- Accession number :
- edsair.doi.dedup.....9ed9f9136c362add35479166711d4cec