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Dietary Grape-Seed Procyanidins Decreased Postweaning Diarrhea by Modulating Intestinal Permeability and Suppressing Oxidative Stress in Rats

Authors :
Peixia Song
Xiaoxiao Wang
Ruoji Zhang
Pingli He
Lulin Tan
Xi Ma
Source :
Journal of Agricultural and Food Chemistry. 59:6227-6232
Publication Year :
2011
Publisher :
American Chemical Society (ACS), 2011.

Abstract

This study was conducted to evaluate the effects of grape-seed procyanidins in controlling weaning diarrhea using a rat model. Weaned rats were fed either the basal diet or basal diet supplemented with either 250 mg/kg grape-seed procyanidins or 2000 mg/kg ZnO. Treated rats had better performance with a reduced incidence of diarrhea (P < 0.05). Both ZnO and grape-seed procyanidins significantly reduced urinary lactulose to mannitol ratios (P < 0.05) and enhanced the mRNA and protein expression of the intestinal mucosal tight junction proteins Ocln/ZO-1 (P < 0.05). Grape-seed procyanidins increased the activities of SOD, GSH-Px, and GSH while decreasing the level of MDA in the intestinal mucosa (P < 0.05). Furthermore, an in vitro investigation revealed that supplementation with grape-seed procyanidins in IEC-6 intestinal epithelial cells significantly enhanced the expression of Ocln/ZO-1 under H(2)O(2)-induced oxidative stress. Collectively, these results indicate that grape-seed procyanidins have the potential to prevent weaning diarrhea by reducing intestinal permeability and improving antioxidant indices.

Details

ISSN :
15205118 and 00218561
Volume :
59
Database :
OpenAIRE
Journal :
Journal of Agricultural and Food Chemistry
Accession number :
edsair.doi.dedup.....9eeba40cfda79921466f1e30ada341df
Full Text :
https://doi.org/10.1021/jf200120y