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ADFP promotes cell proliferation in lung adenocarcinoma via Akt phosphorylation
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2020
- Publisher :
- John Wiley and Sons Inc., 2020.
-
Abstract
- Previously, we identified differentially expressed proteins, including ADFP, between lung adenocarcinoma (LAC) tissue and paired normal bronchioloalveolar epithelium. In this study, we investigated the role of ADFP in LAC. ADFP levels in the serum of patients with lung cancer and benign diseases were measured by enzyme‐linked immunosorbent assays (ELISA). shRNA was used to knock‐down or overexpress ADFP in A549 and NCI‐H1299 cells. The biological function of ADFP and its underlying mechanisms was evaluated in vivo and in vitro. ADFP was highly expressed in the serum of lung cancer patients, especially those with LAC. ADFP promoted cell proliferation and up‐regulated the p‐Akt/Akt ratio in A549 and NCI‐H1299 cells in vitro. Furthermore, in nude mice, ADFP promoted tumour formation with high levels of p‐Akt/Akt, Ki67 and proliferating cell nuclear antigen (PCNA). Similar to the effect of ADFP knock‐down, MK‐2206 (a phosphorylation inhibitor of Akt) reduced A549 and NCI‐H1299 cell proliferation. In ADFP‐overexpressing A549 and NCI‐H1299 cells, proliferation was suppressed by MK‐2206 and returned to the control level. ADFP did not regulate invasion, migration or adhesion in LAC cells. Together, these results suggest that ADFP promotes LAC cell proliferation in vitro and in vivo by increasing Akt phosphorylation level.
- Subjects :
- 0301 basic medicine
Lung Neoplasms
proliferation
ADFP
Mice, Nude
Adenocarcinoma of Lung
Real-Time Polymerase Chain Reaction
phosphorylation of Akt
Perilipin-2
Small hairpin RNA
03 medical and health sciences
Mice
0302 clinical medicine
In vivo
Cell Line, Tumor
Proliferating Cell Nuclear Antigen
medicine
Animals
Humans
Protein kinase B
Mice, Inbred BALB C
Wound Healing
biology
Cell growth
Chemistry
Cell Biology
Original Articles
lung adenocarcinoma
Flow Cytometry
Epithelium
In vitro
Proliferating cell nuclear antigen
030104 developmental biology
medicine.anatomical_structure
A549 Cells
030220 oncology & carcinogenesis
Cancer research
biology.protein
Molecular Medicine
Phosphorylation
Original Article
Female
Proto-Oncogene Proteins c-akt
Subjects
Details
- Language :
- English
- ISSN :
- 15824934 and 15821838
- Volume :
- 25
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....9f0c945d6b6bc8df6c51fbb6b3c9e42c