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Manufacturing and banking canine adipose-derived mesenchymal stem cells for veterinary clinical application
- Source :
- BMC Veterinary Research, Vol 17, Iss 1, Pp 1-11 (2021), BMC Veterinary Research
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Background Mesenchymal stem cells (MSCs) have generated a great amount of interest in recent years as a novel therapeutic application for improving the quality of pet life and helping them free from painful conditions and diseases. It has now become critical to address the challenges related to the safety and efficacy of MSCs expanded in vitro. In this study, we establish a standardized process for manufacture of canine adipose-derived MSCs (AD-MSCs), including tissue sourcing, cell isolation and culture, cryopreservation, thawing and expansion, quality control and testing, and evaluate the safety and efficacy of those cells for clinical applications. Results After expansion, the viability of AD-MSCs manufactured under our standardized process was above 90 %. Expression of surface markers and differentiation potential was consistent with ISCT standards. Sterility, mycoplasma, and endotoxin tests were consistently negative. AD-MSCs presented normal karyotype, and did not form in vivo tumors. No adverse events were noted in the case treated with intravenously AD-MSCs. Conclusions Herein we demonstrated the establishment of a feasible bioprocess for manufacturing and banking canine AD-MSCs for veterinary clinical use.
- Subjects :
- Male
Quality Control
Veterinary medicine
Sterility
Carcinogenicity Tests
Adipose
Cell Culture Techniques
Adipose tissue
Cell Separation
Mice, SCID
Tissue Banks
Mesenchymal Stem Cell Transplantation
Cryopreservation
Cell therapy
Canine
Parvoviridae Infections
Parvovirus
03 medical and health sciences
0302 clinical medicine
Dogs
In vivo
Medicine
Animals
Cell isolation
Bioprocess
030304 developmental biology
0303 health sciences
lcsh:Veterinary medicine
General Veterinary
business.industry
Mesenchymal stem cell
General Medicine
Leukopenia
Banking
Adipose Tissue
030220 oncology & carcinogenesis
Mesenchymal stem cells
lcsh:SF600-1100
Female
business
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 17466148
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Veterinary Research
- Accession number :
- edsair.doi.dedup.....9f3903aaee2046199c4541fec383e38f