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A Single Cysteine Residue in the Translocation Pathway of the Mitosomal ADP/ATP Carrier from Cryptosporidium parvum Confers a Broad Nucleotide Specificity
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 21; Issue 23; Pages: 8971, International Journal of Molecular Sciences, Vol 21, Iss 8971, p 8971 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- Cryptosporidiumparvum is a clinically important eukaryotic parasite that causes the disease cryptosporidiosis, which manifests with gastroenteritis-like symptoms. The protist has mitosomes, which are organelles of mitochondrial origin that have only been partially characterized. The genome encodes a highly reduced set of transport proteins of the SLC25 mitochondrial carrier family of unknown function. Here, we have studied the transport properties of one member of the C. parvum carrier family, demonstrating that it resembles the mitochondrial ADP/ATP carrier of eukaryotes. However, this carrier has a broader substrate specificity for nucleotides, transporting adenosine, thymidine, and uridine di- and triphosphates in contrast to its mitochondrial orthologues, which have a strict substrate specificity for ADP and ATP. Inspection of the putative translocation pathway highlights a cysteine residue, which is a serine in mitochondrial ADP/ATP carriers. When the serine residue is replaced by cysteine or larger hydrophobic residues in the yeast mitochondrial ADP/ATP carrier, the substrate specificity becomes broad, showing that this residue is important for nucleotide base selectivity in ADP/ATP carriers.
- Subjects :
- 0301 basic medicine
Models, Molecular
Atractyloside
Substrate Specificity
lcsh:Chemistry
Serine
chemistry.chemical_compound
0302 clinical medicine
Nucleotide
adenine nucleotide translocase
substrate specificity and selectivity
lcsh:QH301-705.5
Spectroscopy
Phylogeny
chemistry.chemical_classification
biology
Nucleotides
Adenine nucleotide translocator
General Medicine
Computer Science Applications
Transport protein
Mitochondria
Lactococcus lactis
Biochemistry
substrate binding site
adenine nucleotide translocator
SLC25 mitochondrial carrier family
Protein Translocation Systems
ATP–ADP translocase
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Structure-Activity Relationship
Amino Acid Sequence
Cysteine
Physical and Theoretical Chemistry
Molecular Biology
Cryptosporidium parvum
Organic Chemistry
Mitochondrial carrier
Uridine
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
biology.protein
Mutant Proteins
Bongkrekic Acid
Mitochondrial ADP, ATP Translocases
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 21
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....9f80944635e8775c29508756329c3fb3