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Alteration of S‐adenosylhomocysteine levels affects lignin biosynthesis in switchgrass
- Source :
- Plant Biotechnology Journal
- Publication Year :
- 2018
- Publisher :
- John Wiley and Sons Inc., 2018.
-
Abstract
- Summary Methionine (Met) synthesized from aspartate is a fundamental amino acid needed to produce S‐adenosylmethionine (SAM) that is an important cofactor for the methylation of monolignols. As a competitive inhibitor of SAM‐dependent methylation, the effect of S‐adenosylhomocysteine (SAH) on lignin biosynthesis, however, is still largely unknown in plants. Expression levels of Cystathionine γ‐synthase (PvCGS) and S‐adenosylhomocysteine hydrolase 1 (PvSAHH1) were down‐regulated by RNAi technology, respectively, in switchgrass, a dual‐purpose forage and biofuel crop. The transgenic switchgrass lines were subjected to studying the impact of SAH on lignin biosynthesis. Our results showed that down‐regulation of PvCGS in switchgrass altered the accumulation of aspartate‐derived and aromatic amino acids, reduced the content of SAH, enhanced lignin biosynthesis and stunted plant growth. In contrast, down‐regulation of PvSAHH1 raised SAH levels in switchgrass, impaired the biosynthesis of both guaiacyl and syringyl lignins and therefore significantly increased saccharification efficiency of cell walls. This work indicates that SAH plays a crucial role in monolignol methylation in switchgrass. Genetic regulation of either PvCGS or PvSAHH1 expression in switchgrass can change intracellular SAH contents and SAM to SAH ratios and therefore affect lignin biosynthesis. Thus, our study suggests that genes involved in Met metabolism are of interest as new valuable targets for cell wall bioengineering in future.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Carbon-Oxygen Lyases
S‐adenosylhomocysteine
Down-Regulation
Plant Science
Biology
Panicum
01 natural sciences
Lignin
Cell wall
03 medical and health sciences
chemistry.chemical_compound
cystathionine γ‐synthase
Biosynthesis
Cell Wall
S‐adenosylhomocysteine hydrolase
Aromatic amino acids
cardiovascular diseases
Amino Acids
Research Articles
chemistry.chemical_classification
Methionine
Panicum virgatum L. (switchgrass)
Adenosylhomocysteinase
fungi
food and beverages
Methylation
Plants, Genetically Modified
S-Adenosylhomocysteine
Amino acid
nervous system diseases
030104 developmental biology
chemistry
Biochemistry
methionine metabolism
Monolignol
Genetic Engineering
Agronomy and Crop Science
lignin biosynthesis
S‐adenosylmethionine
Metabolic Networks and Pathways
010606 plant biology & botany
Biotechnology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14677652 and 14677644
- Volume :
- 16
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Plant Biotechnology Journal
- Accession number :
- edsair.doi.dedup.....9f887d9288a055ec8b7af68ffa6c4706