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R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 9, p e46171 (2012)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.
- Subjects :
- Embryology
Anatomy and Physiology
Mouse
lcsh:Medicine
Gene Expression
Metastasis
Green fluorescent protein
Mice
Bimolecular fluorescence complementation
0302 clinical medicine
Nucleic Acids
Molecular Cell Biology
Basic Cancer Research
Morphogenesis
Fluorescence microscope
lcsh:Science
Cells, Cultured
0303 health sciences
Multidisciplinary
Stem Cells
Cell Differentiation
Intracellular vesicle
Animal Models
Fluorescence
Cellular Structures
Cell biology
Oncology
Medicine
Membranes and Sorting
Cellular Types
Genetic Engineering
Cell Division
Research Article
Biotechnology
Cell Physiology
Green Fluorescent Proteins
Mitosis
Mice, Transgenic
Biology
03 medical and health sciences
Model Organisms
Genetics
Cancer Genetics
Animals
Alleles
030304 developmental biology
Reporter gene
Tissue Engineering
Integrases
lcsh:R
reporter gene, transgene
Molecular biology
Transplantation
lcsh:Q
mCherry
Organism Development
Animal Genetics
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....9f8c97e4cbc00c9ff72033175178fa7b