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Induction of myogenic differentiation in human rhabdomyosarcoma cells by ionising radiation, N,N-dimethylformamide and their combination
- Source :
- Scopus-Elsevier, British Journal of Cancer
-
Abstract
- Differentiation-inducing ability of gamma-radiation, N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells. Radiation appeared to act by inducing de novo differentiated elements. N,N-dimethylformamide was able to induce an increased myosin expression, but did not affect multinuclear cell proportion. The combined treatment (ionising radiation and N,N-dimethylformamide) resulted in an additive increase in the proportion of myosin-positive cells, approaching 25-35%, but de novo differentiated elements were not increased above the levels obtained with irradiation alone. Images Figure 2
- Subjects :
- Cancer Research
Lung Neoplasms
medicine.medical_treatment
Cellular differentiation
Cell
Clone (cell biology)
Biology
Ionizing radiation
Rhabdomyosarcoma
Myosin
Tumor Cells, Cultured
medicine
Humans
business.industry
Muscles
Cell Differentiation
Dimethylformamide
Dose-Response Relationship, Radiation
medicine.disease
Molecular biology
In vitro
Pulmonary Alveoli
Radiation therapy
medicine.anatomical_structure
Oncology
Gamma Rays
Nuclear medicine
business
Research Article
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier, British Journal of Cancer
- Accession number :
- edsair.doi.dedup.....9fa2e2d7e822d2de6a300823dee965fe