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Amaryllidaceae Alkaloid Cherylline Inhibits the Replication of Dengue and Zika Viruses
- Source :
- Antimicrobial Agents and Chemotherapy
- Publication Year :
- 2021
-
Abstract
- Dengue fever, caused by dengue virus (DENV), is the most prevalent arthropod-borne viral disease and is endemic in many tropical and subtropical parts of the world, with an increasing incidence in temperate regions. The closely related flavivirus Zika virus (ZIKV) can be transmitted vertically in utero and causes congenital Zika syndrome and other birth defects. In adults, ZIKV is associated with Guillain-Barré syndrome. There are no approved antiviral therapies against either virus. Effective antiviral compounds are urgently needed. Amaryllidaceae alkaloids (AAs) are a specific class of nitrogen-containing compounds produced by plants of the Amaryllidaceae family with numerous biological activities. Recently, the AA lycorine was shown to present strong antiflaviviral properties. Previously, we demonstrated that Crinum jagus contained lycorine and several alkaloids of the cherylline, crinine, and galanthamine types with unknown antiviral potential. In this study, we explored their biological activities. We show that C. jagus crude alkaloid extract inhibited DENV infection. Among the purified AAs, cherylline efficiently inhibited both DENV (50% effective concentration [EC50], 8.8 μM) and ZIKV replication (EC50, 20.3 μM) but had no effect on HIV-1 infection. Time-of-drug-addition and -removal experiments identified a postentry step as the one targeted by cherylline. Consistently, using subgenomic replicons and replication-defective genomes, we demonstrate that cherylline specifically hinders the viral RNA synthesis step but not viral translation. In conclusion, AAs are an underestimated source of antiflavivirus compounds, including the effective inhibitor cherylline, which could be optimized for new therapeutic approaches.
- Subjects :
- Adult
viruses
cherylline
Dengue virus
Virus Replication
alkaloids
medicine.disease_cause
Antiviral Agents
Virus
Zika virus
Dengue fever
Dengue
03 medical and health sciences
chemistry.chemical_compound
antivirals
flavivirus
flaviviru
medicine
Humans
Pharmacology (medical)
Amaryllidaceae Alkaloids
030304 developmental biology
Pharmacology
0303 health sciences
dengue virus
biology
Zika Virus Infection
030306 microbiology
Viral translation
Amaryllidaceae
Zika Virus
Isoquinolines
biology.organism_classification
medicine.disease
Lycorine
alkaloid
Virology
antiviral
3. Good health
Zika viru
dengue viru
Flavivirus
Infectious Diseases
chemistry
lycorine
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Antimicrobial Agents and Chemotherapy
- Accession number :
- edsair.doi.dedup.....9fadbc8bd255bd19fef9260a396e1362