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Vanillin augments liver regeneration effectively in Thioacetamide induced liver fibrosis rat model
- Source :
- Life sciences. 286
- Publication Year :
- 2021
-
Abstract
- This study has been designed to investigate the role of vanillin either as prophylaxis or treatment in liver regeneration augmentation and liver fibrosis regression in thioacetamide (TAA) induced liver damage.Animals were injected with TAA to induce liver injury (200mg/kg twice weekly) for 8 weeks. In vanillin prophylaxis group; rats were administered vanillin (100 mg/Kg; IP, daily) from day 1 of TAA injection for 8 weeks. In vanillin treatment group; rats were confronted with the same dose of TAA injection for 8 weeks then treated with vanillin (100 mg/Kg, IP, daily) for 4 weeks. ALT, AST activities, serum albumin, hepatic GSH, MDA, HGF, VEGF, IL-6 and TNF-α levels were measured and also, MMP-2, TIMP-1 and cyclin D gene expression were determined. Liver sections were stained with HE and Sirius red and immunostained for Ki-67 and α-SMA for histological and immunohistological changes analysis.Vanillin improved liver function and histology. Also, showed a remarkable increase in hepatic HGF and VEGF level, and up-regulation of cyclin D1 expression accompanied by a significant up-regulation of MMP-2 and down- regulation of TIMP-1. All these effects were accompanied by TNF-α, IL-6 and oxidative stress significant attenuation.In conclusion, vanillin enhanced liver regeneration in TAA induced liver damage model; targeting growth factors (HGF, VEGF) and cellular proliferation marker cyclin D1. As well as stimulating fibrosis regression by inhibition of ECM accumulation and enhancing its degradation.
- Subjects :
- Liver Cirrhosis
Male
Cyclin D
Serum albumin
Pharmacology
Thioacetamide
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
Cyclin D1
Fibrosis
medicine
Animals
General Pharmacology, Toxicology and Pharmaceutics
Rats, Wistar
Cell Proliferation
Liver injury
biology
General Medicine
Glutathione
medicine.disease
Liver regeneration
Liver Regeneration
Rats
Oxidative Stress
chemistry
Liver
Benzaldehydes
biology.protein
Intercellular Signaling Peptides and Proteins
Subjects
Details
- ISSN :
- 18790631
- Volume :
- 286
- Database :
- OpenAIRE
- Journal :
- Life sciences
- Accession number :
- edsair.doi.dedup.....9fb8fed258547f91393ec91947d1ed92