Back to Search
Start Over
The acidic C-terminus of vaccinia virus I3 single-strand binding protein promotes proper assembly of DNA–protein complexes
- Source :
- Virology. 489:212-222
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The vaccinia virus I3L gene encodes a single-stranded DNA binding protein (SSB) that is essential for virus DNA replication and is conserved in all Chordopoxviruses. The I3 protein contains a negatively charged C-terminal tail that is a common feature of SSBs. Such acidic tails are critical for SSB-dependent replication, recombination and repair. We cloned and purified variants of the I3 protein, along with a homolog from molluscum contagiosum virus, and tested how the acidic tail affected DNA–protein interactions. Deleting the C terminus of I3 enhanced the affinity for single-stranded DNA cellulose and gel shift analyses showed that it also altered the migration of I3-DNA complexes in agarose gels. Microinjecting an antibody against I3 into vaccinia-infected cells also selectively inhibited virus replication. We suggest that this domain promotes cooperative binding of I3 to DNA in a way that would maintain an open DNA configuration around a replication site.
- Subjects :
- DNA Replication
Gene Expression Regulation, Viral
0301 basic medicine
HMG-box
Amino Acid Motifs
Vaccinia virus
Biology
Single-stranded binding protein
Viral Proteins
03 medical and health sciences
Replication factor C
SeqA protein domain
Virology
Vaccinia
Humans
Single-strand DNA binding protein
Replication protein A
Single-strand DNA-binding protein
Molluscum contagiosum virus
Ter protein
DNA replication
Molecular biology
DNA-Binding Proteins
030104 developmental biology
DNA, Viral
biology.protein
I3L
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 489
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....9fba3ed7cff57ac9fab518d794f51ea2
- Full Text :
- https://doi.org/10.1016/j.virol.2015.12.020