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The Yersiniabactin Transport System Is Critical for the Pathogenesis of Bubonic and Pneumonic Plague
- Source :
- Infection and Immunity. 78:2045-2052
- Publication Year :
- 2010
- Publisher :
- American Society for Microbiology, 2010.
-
Abstract
- Iron acquisition from the host is an important step in the pathogenic process. While Yersinia pestis has multiple iron transporters, the yersiniabactin (Ybt) siderophore-dependent system plays a major role in iron acquisition in vitro and in vivo . In this study, we determined that the Ybt system is required for the use of iron bound by transferrin and lactoferrin and examined the importance of the Ybt system for virulence in mouse models of bubonic and pneumonic plague. Y. pestis mutants unable to either transport Ybt or synthesize the siderophore were both essentially avirulent via subcutaneous injection (bubonic plague model). Surprisingly, via intranasal instillation (pneumonic plague model), we saw a difference in the virulence of Ybt biosynthetic and transport mutants. Ybt biosynthetic mutants displayed an ∼24-fold-higher 50% lethal dose (LD 50 ) than transport mutants. In contrast, under iron-restricted conditions in vitro , a Ybt transport mutant had a more severe growth defect than the Ybt biosynthetic mutant. Finally, a Δ pgm mutant had a greater loss of virulence than the Ybt biosynthetic mutant, indicating that the 102-kb pgm locus encodes a virulence factor, in addition to Ybt, that plays a role in the pathogenesis of pneumonic plague.
- Subjects :
- Pneumonic plague
Siderophore
Virulence Factors
Yersinia pestis
Iron
Immunology
Mutant
Virulence
Biology
Microbiology
Yersiniabactin
Virulence factor
Lethal Dose 50
Mice
chemistry.chemical_compound
Phenols
medicine
Animals
Plague
Yersiniosis
medicine.disease
biology.organism_classification
Molecular Pathogenesis
Survival Analysis
Thiazoles
Infectious Diseases
chemistry
Female
Parasitology
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 78
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....a01e8c2ef5042d270261ca7f42968441