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MicroRNA-26a–interleukin (IL)-6–IL-17 axis regulates the development of non-alcoholic fatty liver disease in a murine model
- Source :
- Clinical and Experimental Immunology. 187:174-184
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Summary Non-alcoholic fatty liver disease (NAFLD) is a hepatic presentation of obesity and metabolic syndrome. MicroRNA 26a (Mir-26a) has been reported to play functions in cellular differentiation, cell growth, cell apoptosis and metastasis. A recent paper indicated that Mir-26a regulated insulin sensitivity and metabolism of glucose and lipids. However, the role of Mir-26a in NAFLD still needs to be investigated further. In our current study, vectors encoding pre-Mir-26a (LV-26a) and an empty lentiviral vector (LV-Con) delivered approximately 2 × 107 transforming units of recombinant lentivirus were injected into mice through the tail vein. LV-26a-infected mice were protected from glucose dysmetabolism and showed markedly decreased total liver weight, hepatic triglyceride deposition and serum alanine transaminase (ALT) concentration when compared with LV-Con-treated mice. LV-26a-treated mice also exhibited decreased infiltration of immune cells in the liver – something attributed to reduce infiltration of T cell receptor (TCR)-γδ+, granulocyte-differentiation antigen-1 (Gr-1)+ cells and CD11b+ cells. Next, we found that Mir-26a inhibited the expression of interleukin (IL)−17 and IL-6 in vivo and in vitro. Furthermore, the decreased expression of IL-17 in the liver tissue induced by Mir-26a was abrogated completely by IL-6 overexpression. The decreased total liver weight, hepatic triglyceride deposition and serum ALT concentration induced by Mir-26a was also abrogated completely by IL-6 over-expression. In conclusion, the Mir-26a–IL-6–IL-17 axis regulates the development of NAFLD in a murine model.
- Subjects :
- Male
0301 basic medicine
T-Lymphocytes
Cellular differentiation
Immunology
Mice
03 medical and health sciences
Non-alcoholic Fatty Liver Disease
medicine
Animals
Humans
Immunology and Allergy
Interleukin 6
biology
Interleukin-6
Cell growth
Interleukin-17
Lentivirus
Fatty liver
Interleukin
Alanine Transaminase
Receptors, Antigen, T-Cell, gamma-delta
Original Articles
Genetic Therapy
Lipid Metabolism
medicine.disease
Mice, Inbred C57BL
Disease Models, Animal
MicroRNAs
030104 developmental biology
Liver
Alanine transaminase
biology.protein
Interleukin 17
Insulin Resistance
Metabolic syndrome
Subjects
Details
- ISSN :
- 13652249 and 00099104
- Volume :
- 187
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Immunology
- Accession number :
- edsair.doi.dedup.....a02be779c6cfeb6479b2f8ca447d54e4
- Full Text :
- https://doi.org/10.1111/cei.12838