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Tyramine induces dynamic RNP granule remodeling and translation activation in the Drosophila brain
- Source :
- eLife, eLife, Vol 10 (2021), eLife, 2021, 10, pp.e65742. ⟨10.7554/eLife.65742⟩, eLife, eLife Sciences Publication, 2021, 10, pp.e65742. ⟨10.7554/eLife.65742⟩
- Publication Year :
- 2021
- Publisher :
- eLife Sciences Publications, Ltd, 2021.
-
Abstract
- Ribonucleoprotein (RNP) granules are dynamic condensates enriched in regulatory RNA binding proteins (RBPs) and RNAs under tight spatiotemporal control. Extensive recent work has investigated the molecular principles underlying RNP granule assembly, unraveling that they form through the self-association of RNP components into dynamic networks of interactions. How endogenous RNP granules respond to external stimuli to regulate RNA fate is still largely unknown. Here, we demonstrate through high-resolution imaging of intact Drosophila brains that Tyramine induces a reversible remodeling of somatic RNP granules characterized by the decondensation of granule-enriched RBPs (e.g. Imp/ZBP1/IGF2BP) and helicases (e.g. Me31B/DDX-6/Rck). Furthermore, our functional analysis reveals that Tyramine signals both through its receptor TyrR and through the calcium-activated kinase CamkII to trigger RNP component decondensation. Finally, we uncover that RNP granule remodeling is accompanied by the rapid and specific translational activation of associated mRNAs. Thus, this work sheds new light on the mechanisms controlling cue-induced rearrangement of physiological RNP condensates.
- Subjects :
- ZBP1
QH301-705.5
Science
translation
RNA-binding protein
General Biochemistry, Genetics and Molecular Biology
RNP condensates
03 medical and health sciences
0302 clinical medicine
Ca2+/calmodulin-dependent protein kinase
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Biology (General)
030304 developmental biology
Ribonucleoprotein
0303 health sciences
D. melanogaster
General Immunology and Microbiology
biology
Chemistry
General Neuroscience
Helicase
RNA
Translation (biology)
Cell Biology
General Medicine
neuron
Cell biology
live-imaging
biology.protein
Medicine
Translational Activation
Drosophila
RNA binding proteins
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 2050084X
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....a035eaf726cb358c9ae6c9da324e6b5a