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Protective effects of baicalin on carbon tetrachloride induced liver injury by activating PPARγ and inhibiting TGFβ1
- Source :
- Pharmaceutical Biology. 49:38-45
- Publication Year :
- 2010
- Publisher :
- Informa UK Limited, 2010.
-
Abstract
- Traditional Chinese herbal medicines have attracted considerable attention in many countries with treatment of several end-stage liver diseases.The present study investigated the protective effects of baicalin on hepatotoxicity and hepatic fibrosis and explored the role of transforming growth factor β1 (TGFβ1) and peroxisome proliferator activated receptors γ (PPARγ) on the rat liver injury model.The rat liver injury model was introduced by subcutaneous injection of carbon tetrachloride (CCl(4)) for 8 weeks. At week 5, rats were treated with baicalin of different doses or silymarin. Detection of biochemical indicators, histological analysis, and enzyme-linked immunosorbent assays were employed to evaluate severity of liver inflammation, and western blotting and RT-PCR assay were performed to evaluate TGFβ1 and PPARγ pathway related proteins and gene expression.The administration of baicalin could significantly improve histological changes of CCl(4) treated rat livers and return biochemical indicators for liver injury to nearly baseline level. In addition, the increased expression of TGFβ1 was markedly suppressed by baicalin, and decreased expression of PPARγ was also dramatically elevated by baicalin as well. The hepatoprotective effects of baicalin may be conferred by elevating the level of PPARγ contributing to down-regulation of TGFβ1 signaling pathway and suppression of hepatic stellate cell activation.The studies demonstrated that baicalin is a potent and promising antifibrotic drug in the treatment of hepatic fibrosis.
- Subjects :
- Liver Cirrhosis
Male
medicine.medical_specialty
Blotting, Western
Pharmaceutical Science
Peroxisome proliferator-activated receptor
CCL4
Context (language use)
Severity of Illness Index
Rats, Sprague-Dawley
Transforming Growth Factor beta1
chemistry.chemical_compound
Internal medicine
Drug Discovery
Animals
Medicine
Medicine, Chinese Traditional
Carbon Tetrachloride
Flavonoids
Pharmacology
Liver injury
chemistry.chemical_classification
Dose-Response Relationship, Drug
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Liver Diseases
General Medicine
medicine.disease
Rats
PPAR gamma
Disease Models, Animal
Endocrinology
Gene Expression Regulation
Complementary and alternative medicine
chemistry
Carbon tetrachloride
Molecular Medicine
Hepatic fibrosis
business
Baicalin
Silymarin
Transforming growth factor
Subjects
Details
- ISSN :
- 17445116 and 13880209
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical Biology
- Accession number :
- edsair.doi.dedup.....a055e80fbca252cb23ec963708d96ac1