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Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus
- Source :
- PLoS ONE, Vol 11, Iss 4, p e0152870 (2016), PLoS ONE, PLOS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- The Atlantic salmon (Salmo salar) and African bichir (Polypterus senegalus) are both actinopterygian fish species that continuously replace their teeth without the involvement of a successional dental lamina. Instead, they share the presence of a middle dental epithelium: an epithelial tier enclosed by inner and outer dental epithelium. It has been hypothesized that this tier could functionally substitute for a successional dental lamina and might be a potential niche to house epithelial stem cells involved in tooth cycling. Therefore, in this study we performed a BrdU pulse chase experiment on both species to (1) determine the localization and extent of proliferating cells in the dental epithelial layers, (2) describe cell dynamics and (3) investigate if label-retaining cells are present, suggestive for the putative presence of stem cells. Cells proliferate in the middle dental epithelium, outer dental epithelium and cervical loop at the lingual side of the dental organ to form a new tooth germ. Using long chase times, both in S. salar (eight weeks) and P. senegalus (eight weeks and twelve weeks), we could not reveal the presence of label-retaining cells in the dental organ. Immunostaining of P. senegalus dental organs for the transcription factor Sox2, often used as a stem cell marker, labelled cells in the zone of outer dental epithelium which grades into the oral epithelium (ODE transition zone) and the inner dental epithelium of a successor only. The location of Sox2 distribution does not provide evidence for epithelial stem cells in the dental organ and, more specifically, in the middle dental epithelium. Comparison of S. salar and P. senegalus reveals shared traits in tooth cycling and thus advances our understanding of the developmental mechanism that ensures lifelong replacement.
- Subjects :
- 0301 basic medicine
Pathology
LOWER JAW DENTITION
Teeth
Physiology
Digestive Physiology
Cervical loop
lcsh:Medicine
Stem cell marker
Epithelium
TEETH
0302 clinical medicine
Animal Cells
ATLANTIC SALMON
Medicine and Health Sciences
Morphogenesis
Stem Cell Niche
lcsh:Science
Staining
Multidisciplinary
biology
Stem Cells
Bromodeoxyuridine Labeling
Fishes
DERMAL SKELETON
Dental lamina
Polypterus senegalus
REPLACEMENT
VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471
medicine.anatomical_structure
VDP::Landbruks- og fiskerifag: 900::Fiskerifag: 920
Stem cell
Anatomy
Cellular Types
STEM-CELLS
Research Article
medicine.medical_specialty
Salmo salar
ZEBRAFISH DANIO-RERIO
Research and Analysis Methods
03 medical and health sciences
SOX2
stomatognathic system
REGENERATION
medicine
DENTAL LAMINA
VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Cell biology: 471
Animals
Dentition
Molecular Biology Techniques
Molecular Biology
Reduced enamel epithelium
Cell Proliferation
lcsh:R
Biology and Life Sciences
Epithelial Cells
Cell Biology
biology.organism_classification
Nuclear Staining
stomatognathic diseases
030104 developmental biology
Biological Tissue
Jaw
Cell Labeling
Specimen Preparation and Treatment
ADULT STEM
lcsh:Q
VDP::Agriculture and fisheries science: 900::Fisheries science: 920
Tooth
Digestive System
Head
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a07d34a923c196d7bd571d48396c9495