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Community analysis of a full-scale anaerobic bioreactor treating paper mill wastewater
- Source :
- Systematic and Applied Microbiology, 28(2), 175-185, Systematic and Applied Microbiology 28 (2005) 2
- Publication Year :
- 2005
-
Abstract
- To get insight into the microbial community of an Upflow Anaerobic Sludge Blanket reactor treating paper mill wastewater, conventional microbiological methods were combined with 16S rRNA gene analyses. Particular attention was paid to microorganisms able to degrade propionate or butyrate in the presence or absence of sulphate. Serial enrichment dilutions allowed estimating the number of microorganisms per ml sludge that could use butyrate with or without sulphate (10(5)), propionate without sulphate (10(6)), or propionate and sulphate (10(8)). Quantitative RNA dotblot hybridisation indicated that Archaea were two-times more abundant in the microbial community of anaerobic sludge than Bacteria. The microbial community composition was further characterised by 16S rRNA-gene-targeted Denaturing Gradient Gel Electrophoresis (DGGE) fingerprinting, and via cloning and sequencing of dominant amplicons from the bacterial and archaeal patterns. Most of the nearly full length (approximately 1.45 kb) bacterial 16S rRNA gene sequences showed less than 97% similarity to sequences present in public databases, in contrast to the archaeal clones (approximately. 1.3 kb) that were highly similar to known sequences. While Methanosaeta was found as the most abundant genus, also Crenarchaeote-relatives were identified. The microbial community was relatively stable over a period of 3 years (samples taken in July 1999, May 200 1, March 2002 and June 2002) as indicated by the high similarity index calculated from DGGE profiles (81.9 +/- 2.7% for Bacteria and 75.1 +/- 3.1% for Archaea). 16S rRNA gene sequence analysis indicated the presence of unknown and yet uncultured microorganisms, but also showed that known sulphate-reducing bacteria and syntrophic fatty acid-oxidising microorganisms dominated the enrichments. (c) 2004 Elsevier GmbH. All rights reserved.
- Subjects :
- DNA, Bacterial
Paper
granular sludge
Microorganism
Molecular Sequence Data
Industrial Waste
Biology
DNA, Ribosomal
Waste Disposal, Fluid
Applied Microbiology and Biotechnology
Microbiology
Methanosaeta
reactor
Bioreactors
Microbiologie
RNA, Ribosomal, 16S
Anaerobiosis
genes
microorganisms
hybridization
Ecosystem
Ecology, Evolution, Behavior and Systematics
chemistry.chemical_classification
WIMEK
propionate-oxidizing bacteria
Bacteria
Sewage
ribosomal-rna
Ribosomal RNA
16S ribosomal RNA
biology.organism_classification
Archaea
quantification
DNA, Archaeal
chemistry
Microbial population biology
microbial diversity
Propionate
ecology
Temperature gradient gel electrophoresis
Subjects
Details
- Language :
- English
- ISSN :
- 07232020
- Database :
- OpenAIRE
- Journal :
- Systematic and Applied Microbiology, 28(2), 175-185, Systematic and Applied Microbiology 28 (2005) 2
- Accession number :
- edsair.doi.dedup.....a08803dc41018991281ac026ec03607f