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Evolutionary conservation of the dystrophin central rod domain
- Source :
- Biochemical Journal. 287:755-759
- Publication Year :
- 1992
- Publisher :
- Portland Press Ltd., 1992.
-
Abstract
- Dystrophin cDNA fragments encoding the C-terminal repeats of the central rod region have been expressed as fusion proteins. The polyclonal antisera raised to the purified fusion proteins have been characterized and neither antiserum cross-reacted with dystrophin-related protein. Antisera detected dystrophin with molecular mass close to that of the human in all terrestrial vertebrates and amphibia studied. Experiments with antisera to the N-terminal region of the dystrophin rod confirmed that epitopes to the rod region were conserved during this evolutionary period and the length of this domain remained unaltered.
- Subjects :
- musculoskeletal diseases
congenital, hereditary, and neonatal diseases and abnormalities
Recombinant Fusion Proteins
Blotting, Western
Biology
Biochemistry
Epitope
Conserved sequence
Dystrophin
Epitopes
Species Specificity
Complementary DNA
Animals
Humans
Molecular Biology
Antiserum
Molecular mass
Cell Biology
musculoskeletal system
Biological Evolution
Fusion protein
Molecular biology
Polyclonal antibodies
biology.protein
Electrophoresis, Polyacrylamide Gel
Research Article
Subjects
Details
- ISSN :
- 14708728 and 02646021
- Volume :
- 287
- Database :
- OpenAIRE
- Journal :
- Biochemical Journal
- Accession number :
- edsair.doi.dedup.....a0b077b2aee5aaaaf9798e4ee7606541
- Full Text :
- https://doi.org/10.1042/bj2870755