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Lack of anticipatory behavior in Gpr88 knockout mice showed by automatized home cage phenotyping
- Source :
- Genes, Brain and Behavior, Genes, Brain and Behavior, 2019, ⟨10.1111/gbb.12473⟩
- Publication Year :
- 2017
-
Abstract
- Mouse models are widely used to understand genetic bases of behavior. Traditional testing typically requires multiple experimental settings, captures only snapshots of behavior, and involves human intervention. The recent development of automated home cage monitoring offers an alternative method to study mouse behavior in their familiar and social environment, and over weeks. Here we used the IntelliCage system to test this approach for mouse phenotyping, and studied mice lacking the Gpr88 that have been extensively studied using standard testing. We monitored mouse behavior over 22 days in 4 different phases. In the free adaptation phase, Gpr88−/− mice showed delayed habituation to the home cage, and increased frequency of same corner returns behavior in their alternation pattern. In the following nose-poke adaptation phase, non-habituation continued, however mutant mice acquired nose-poke conditioning similarly to controls. In the place learning and reversal phase, Gpr88−/− mice developed preference for the water/sucrose corner with some delay, but did not differ from controls for reversal. Finally, in a fixed schedule-drinking phase, control animals showed higher activity during the hour preceding water accessibility, and reduced activity after access to water was terminated. Mutant mice did not show this behavior, revealing lack of anticipatory behavior. Our data therefore confirm hyperactivity, non-habituation and altered exploratory behaviors that were reported previously. Learning deficits described in other settings were barely detectable, and a novel phenotype was discovered. Home cage monitoring therefore extends previous findings and reveals yet another facet of GPR88 function that deserves further investigation.
- Subjects :
- 0301 basic medicine
Perseveration
perseveration
Biology
Motor Activity
Article
Receptors, G-Protein-Coupled
03 medical and health sciences
Behavioral Neuroscience
non-habituation
Mice
0302 clinical medicine
Genetics
medicine
Animals
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Habituation
GPR88
female mice
intellicage system
Alternative methods
Mice, Knockout
anticipatory behavior
learning
Behavior, Animal
long-term phenotyping
hyperactivity
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
automated
Phenotype
Neurology
Knockout mouse
Exploratory Behavior
Home cage
Conditioning
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
medicine.symptom
Neuroscience
Behavior Observation Techniques
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 1601183X and 16011848
- Volume :
- 17
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Genes, brain, and behavior
- Accession number :
- edsair.doi.dedup.....a0ce1b55ccace939c47bf36d51f35a22