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Discs large (Dlg1) complexes in lymphocyte activation

Authors :
J. Bernabe Ortiz
Albert C. Shaw
Shahrooz Rabizadeh
Ramnik J. Xavier
Kazuhiro Ishiguro
Marco Lopez-Ilasaca
Heather Wachtel
Niko Andre
Wojciech Swat
David G. Morris
Brian Seed
Source :
The Journal of Cell Biology
Publication Year :
2004
Publisher :
Rockefeller University Press, 2004.

Abstract

T cell antigen recognition involves the formation of a structured interface between antigen-presenting and T cells that facilitates the specific transmission of activating and desensitizing stimuli. The molecular machinery that organizes the signaling molecules and controls their disposition in response to activation remains poorly understood. We show here that in T cells Discs large (Dlg1), a PDZ domain-containing protein, is recruited upon activation to cortical actin and forms complexes with early participants in T cell activation. Transient overexpression of Dlg1 attenuates basal and Vav1-induced NFAT reporter activation. Reduction of Dlg1 expression by RNA interference enhances both CD3- and superantigen-mediated NFAT activation. Attenuation of antigen receptor signaling appears to be a complex, highly orchestrated event that involves the mutual segregation of important elements of the early signaling complex.

Details

ISSN :
15408140 and 00219525
Volume :
166
Database :
OpenAIRE
Journal :
Journal of Cell Biology
Accession number :
edsair.doi.dedup.....a0dd5faaa60a95735e04d96860f0cc1d
Full Text :
https://doi.org/10.1083/jcb.200309044