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Ancient DNA of Metagonimus yokogawai Recovered from Joseon Period Human Remains Newly Discovered at Goryeong County in South Korea
- Source :
- Acta Parasitologica. 67:539-545
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Metagonimiasis, commonly seen in East Asian countries, is a parasitic disorder caused by definitive hosts’ ingestion of undercooked freshwater fishes. Recently, genetic analysis has proved 28S rRNA and cytochrome c oxidase subunit I (COI) mtDNA gene to be a successful marker differentiating species of the genus Metagonimus. In the present study, using specimens from the newly discovered Joseon Dynasty human remains of Goryeong, we obtained updated genetic data on genus Metagonimus, which was also prevalent during the Joseon period. The ancient DNA (aDNA) was retrieved from the coprolite sample of the seventeenth century, half-mummified individual discovered at Goryeong Country, South Korea. Cloning and sequencing were performed on PCR-amplified amplicons for M. yokogawai 28S rRNA and COI mtDNA gene. The consensus sequences were used for species identification and phylogenetic analysis using NCBI/BLAST and MEGA X software. Based on the COI mtDNA gene region, the Goryeong sequence was confirmed as belonging to M. yokogawai, as it was shown to form a separate cluster with other M. yokogawai taxa that are distinct also from M. takahashii and M. miyatai. In a series of our genetic analyses on genus Metagonimus using samples retrieved from Joseon-period cases, aDNA sequences of genus Metagonimus revealed in South Korea thus far are those of M. yokogawai, but not of M. miyatai or M. takahashii yet.
- Subjects :
- Mitochondrial DNA
Phylogenetic tree
ved/biology
ved/biology.organism_classification_rank.species
Cytochrome c oxidase subunit I
Trematode Infections
Metagonimus yokogawai
Biology
medicine.disease
Genetic analysis
Body Remains
Ancient DNA
Evolutionary biology
Metagonimiasis
28S ribosomal RNA
Republic of Korea
parasitic diseases
medicine
Animals
Humans
Parasitology
DNA, Ancient
Heterophyidae
Phylogeny
Subjects
Details
- ISSN :
- 18961851 and 12302821
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Acta Parasitologica
- Accession number :
- edsair.doi.dedup.....a128ea7fff35955fa658d869908bb733